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Literature summary extracted from

  • Cao, L.; Xie, L.; Xue, X.; Tan, H.; Liu, Y.; Zhou, S.
    Purification and characterization of alginate lyase from Streptomyces species strain A5 isolated from banana rhizosphere (2007), J. Agric. Food Chem., 55, 5113-5117.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.2.3 EDTA enhances activity Streptomyces sp.

Application

EC Number Application Comment Organism
4.2.2.3 agriculture the alginate oligomers prepared by the lyase from Streptomyces sp. A5 show growth-promoting activity on the roots of banana plantlets. Encapsulation method using alginate microbeads to inoculate beneficial streptomycete strains might be beneficial to the root growth of banana plantlets Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 Cu2+ 2 mM Streptomyces sp.
4.2.2.3 Fe3+ 2 mM Streptomyces sp.
4.2.2.3 Hg2+ 2 mM Streptomyces sp.
4.2.2.3 SDS 0.05 M Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.3 additional information
-
 additional information Km for sodium alginate is 0.13 mg/ml Streptomyces sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.3 extracellular
-
 Streptomyces sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 32000
-
 x * 32000, SDS-PAGE Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.2.3 alginate Streptomyces sp. the alginate oligomers prepared by the lyase show growth-promoting activity on the roots of banana plantlets (Streptomyces sp. A5 is isolated from banana rhizosphere) ?
-
 ?
4.2.2.3 alginate Streptomyces sp. A5 the alginate oligomers prepared by the lyase show growth-promoting activity on the roots of banana plantlets (Streptomyces sp. A5 is isolated from banana rhizosphere) ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Streptomyces sp.
-
 isolated from banana rhizosphere
-
4.2.2.3 Streptomyces sp. A5
-
 isolated from banana rhizosphere
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3
-
 Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alginate the alginate oligomers prepared by the lyase show growth-promoting activity on the roots of banana plantlets (Streptomyces sp. A5 is isolated from banana rhizosphere) Streptomyces sp. ?
-
 ?
4.2.2.3 alginate substrate specificity for polyguluronate units in the alginate molecules Streptomyces sp. ?
-
 ?
4.2.2.3 alginate the alginate oligomers prepared by the lyase show growth-promoting activity on the roots of banana plantlets (Streptomyces sp. A5 is isolated from banana rhizosphere) Streptomyces sp. A5 ?
-
 ?
4.2.2.3 alginate substrate specificity for polyguluronate units in the alginate molecules Streptomyces sp. A5 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 ? x * 32000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 37
-
-
 Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.3 70
-
 92% of the activity is lost after incubation at 70°C and pH 7.5 for 20 min Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7.5
-
-
 Streptomyces sp.