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Literature summary extracted from

  • Duvetter, T.; Fraeye, I.; Sila, D.N.; Verlent, I.; Smout, C.; Hendrickx, M.; Van Loey, A.
    Mode of de-esterification of alkaline and acidic pectin methyl esterases at different pH conditions (2006), J. Agric. Food Chem., 54, 7825-7831.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.11 pectin + H2O Solanum lycopersicum
-
 methanol + pectate
-
 ?
3.1.1.11 pectin + H2O Aspergillus aculeatus
-
 methanol + pectate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.11 Aspergillus aculeatus
-
-
-
3.1.1.11 Solanum lycopersicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.11 further purification of a commercial preparation by gel filtration Aspergillus aculeatus
3.1.1.11 native enzyme partially from fruits by ammonium sulfate fractionation and dialysis Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.11 commercial preparation recombinant enzyme Aspergillus aculeatus
-
3.1.1.11 fruit
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.11 pectin + H2O
-
 Solanum lycopersicum methanol + pectate
-
 ?
3.1.1.11 pectin + H2O
-
 Aspergillus aculeatus methanol + pectate
-
 ?
3.1.1.11 pectin + H2O citrus pectin Solanum lycopersicum methanol + pectate
-
 ?
3.1.1.11 pectin + H2O citrus pectin Aspergillus aculeatus methanol + pectate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.11 pectin methyl esterase
-
 Solanum lycopersicum
3.1.1.11 pectin methyl esterase
-
 Aspergillus aculeatus
3.1.1.11 PME
-
 Solanum lycopersicum
3.1.1.11 PME
-
 Aspergillus aculeatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.11 22
-
 assay at Solanum lycopersicum
3.1.1.11 22
-
 assay at Aspergillus aculeatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.11 80
-
 5 min, partially purified enzyme, inactivation Solanum lycopersicum
3.1.1.11 80
-
 5 min, purified enzyme, inactivation Aspergillus aculeatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.11 additional information
-
 acidic pH optimum Aspergillus aculeatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.11 4.5 8 pH-dependent activity pattern, pH-profile, overview Solanum lycopersicum
3.1.1.11 4.5 8 pH-dependent activity pattern, pH-profile, overview Aspergillus aculeatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.11 Aspergillus aculeatus acidic isoelectric pH
-
 additional information