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Literature summary extracted from

  • Sharma, J.; Singh, A.; Kumar, R.; Mittal, A.
    Partial purification of an alkaline protease from a new strain of Aspergillus oryzae AWT 20 and its enhanced stabilization in entrapped Ca-alginate beads (2006), Internet J. Microbiol., 2, 0000.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.63 additional information stabilization of the enzyme in entrapped Ca-alginate beads, activity maximal (ca. 48%) at 2% (w/v) sodium alginate concentration Aspergillus oryzae

Application

EC Number Application Comment Organism
3.4.21.63 industry immobilization of the enzyme as an important biotechnological aspect Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.63 Hg2+ strongly inhibits Aspergillus oryzae
3.4.21.63 Mn2+ slightly inhibits Aspergillus oryzae
3.4.21.63 additional information not inhibited by Cu2+, Sn2+, Ba2+, Co2+, Li+, benzamidine, beta-ME, cysteine, EDTA and 1,10-phenathroline Aspergillus oryzae
3.4.21.63 NaN3 slightly inhibits Aspergillus oryzae
3.4.21.63 phenylmethylsulfonyl fluoride completely inhibits Aspergillus oryzae
3.4.21.63 TLCK moderately inhibits Aspergillus oryzae
3.4.21.63 TPCK moderately inhibits Aspergillus oryzae
3.4.21.63 Trypsin inhibitor moderately inhibits Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.63 additional information
-
additional information Km for the immobilized enzyme is 1.5fold higher than for the free enzyme Aspergillus oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.63 Ca2+ activates 35% at 1 mM Aspergillus oryzae
3.4.21.63 Mg2+ activates 19% at 1 mM Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.63 33000
-
gel filtration Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.63 Aspergillus oryzae
-
-
-
3.4.21.63 Aspergillus oryzae AWT20
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.63 partially purified by ion exchange chromatography and gel filtration, 30fold Aspergillus oryzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.63 25.7
-
crude extract Aspergillus oryzae
3.4.21.63 750
-
30fold purified enzyme Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.63 casein + H2O active centre of the enzyme contains serine residues Aspergillus oryzae hydrolyzed casein
-
?
3.4.21.63 casein + H2O active centre of the enzyme contains serine residues Aspergillus oryzae AWT20 hydrolyzed casein
-
?

Subunits

EC Number Subunits Comment Organism
3.4.21.63 monomer 1 * 33000, SDS-PAGE Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
3.4.21.63 Alkaline protease
-
Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.63 55
-
immobilized enzyme retains 86% of its activity while the free enzyme retains only 10% Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.63 9.5
-
immobilized enzyme Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.63 5.5 10 immobilized enzyme, shows enhanced stability in acidic as well as alkaline environments in comparison to the free enzyme (stable between pH 7.0-9.0) Aspergillus oryzae