EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.13.3 | additional information | great effect of temperature on the dynamics of the HAMP linker domain, which is a key feature in signal transduction | Mycobacterium tuberculosis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.13.3 | into pQE30 and overexpressed in Escherichia coli M15 | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.13.3 | additional information | thermal transition of HK1 is a two-state process and that of HK2 is a three-state process. Urea denaturation of HK1 and HK2 is a three-state and two-state process, respectively | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.13.3 | Mycobacterium tuberculosis | - |
- |
- |
2.7.13.3 | Mycobacterium tuberculosis H37Rv | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.13.3 | by Ni-NTA column and gel filtration | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.13.3 | histidine kinase | - |
Mycobacterium tuberculosis |
2.7.13.3 | HK1 | - |
Mycobacterium tuberculosis |
2.7.13.3 | HK2 | - |
Mycobacterium tuberculosis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.13.3 | 40 | - |
HK2 is stable up to 40°C and reaches an intermediate state at 60°C. On raising the temperature to 100°C, the second stage of denaturation is observed | Mycobacterium tuberculosis |
2.7.13.3 | 60 | - |
HK1 relatively stable up to 60°C, gets completely denatured at 90°C | Mycobacterium tuberculosis |