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Literature summary extracted from

  • Skowronek, M.; Fiedurek, J.
    Purification and properties of extracellular endoinulinase from Aspergillus niger 20 OSM (2006), Food Technol. Biotechnol., 44, 53-58.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.7 Cd2+ 1 mM, strong inhibition Aspergillus niger
3.2.1.7 Co2+ 1 mM, strong inhibition Aspergillus niger
3.2.1.7 EDTA 1 mM, 83% inhibition Aspergillus niger
3.2.1.7 Fe3+ 1 mM, strong inhibition Aspergillus niger
3.2.1.7 Hg2+ 1 mM, complete inactivation Aspergillus niger
3.2.1.7 Mn2+ 1 mM, complete inactivation Aspergillus niger
3.2.1.7 Ni2+ 1 mM, strong inhibition Aspergillus niger
3.2.1.7 p-chloromercuribenzoate
-
 Aspergillus niger
3.2.1.7 Zn2+ 1 mM, strong inhibition Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.7 extracellular
-
 Aspergillus niger
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.7 Ca2+
-
 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.7 64000
-
 gel filtration Aspergillus niger
3.2.1.7 69000
-
 1 * 69000, SDS-PAGE Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.7 Aspergillus niger
-
 20 OSM
-
3.2.1.7 Aspergillus niger 20 OSM
-
 20 OSM
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.7 glycoprotein carbohydrate content is about 44.5% Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.7
-
 Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.7 culture filtrate
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.7 1158
-
-
 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.7 inulin + H2O during the hydrolysis process, at first longer saccharide chains appear, which are subsequently cut into shorter fragments Aspergillus niger ?
-
 ?
3.2.1.7 inulin + H2O during the hydrolysis process, at first longer saccharide chains appear, which are subsequently cut into shorter fragments Aspergillus niger 20 OSM ?
-
 ?
3.2.1.7 additional information purified inulinase shows no detectable activity toward sucrose and little activity toward raffinose (0.8% of relative enzyme activity on inulin) Aspergillus niger ?
-
 ?
3.2.1.7 additional information purified inulinase shows no detectable activity toward sucrose and little activity toward raffinose (0.8% of relative enzyme activity on inulin) Aspergillus niger 20 OSM ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.7 monomer 1 * 69000, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.7 endoinulinase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.7 55
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.7 55
-
 stable below Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.7 5
-
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.7 4 7 stable Aspergillus niger

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.7 Aspergillus niger chromatofocusing
-
 3.85