EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.21 | Inositol | constant enzyme activation, values of relative activation about 40% | Phlebotomus papatasi |
EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.1 | analysis | comparison of glycolytic and chitinolytic enzyme activities between desert and oasis flies of Phlebotomus papatasi to evaluate potential differences in susceptibility to infection with Leishmania major | Phlebotomus papatasi |
3.2.1.14 | analysis | comparison of glycolytic and chitinolytic enzyme activities between desert and oasis flies of Phlebotomus papatasi to evaluate potential differences in susceptibility to infection with Leishmania major | Phlebotomus papatasi |
3.2.1.21 | analysis | comparison of glycolytic and chitinolytic enzyme activities between desert and oasis flies of Phlebotomus papatasi to evaluate potential differences in susceptibility to infection with Leishmania major | Phlebotomus papatasi |
3.2.1.22 | analysis | comparison of glycolytic and chitinolytic enzyme activities between desert and oasis flies of Phlebotomus papatasi to evaluate potential differences in susceptibility to infection with Leishmania major | Phlebotomus papatasi |
3.2.1.24 | analysis | comparison of glycolytic and chitinolytic enzyme activities between desert and oasis flies of Phlebotomus papatasi to evaluate potential differences in susceptibility to infection with Leishmania major | Phlebotomus papatasi |
3.2.1.25 | analysis | comparison of glycolytic and chitinolytic enzyme activities between desert and oasis flies of Phlebotomus papatasi to evaluate potential differences in susceptibility to infection with Leishmania major | Phlebotomus papatasi |
3.2.1.26 | analysis | comparison of glycolytic and chitinolytic enzyme activities between desert and oasis flies of Phlebotomus papatasi to evaluate potential differences in susceptibility to infection with Leishmania major | Phlebotomus papatasi |
3.2.1.37 | analysis | comparison of glycolytic and chitinolytic enzyme activities between desert and oasis flies of Phlebotomus papatasi to evaluate potential differences in susceptibility to infection with Leishmania major | Phlebotomus papatasi |
3.2.1.38 | analysis | comparison of glycolytic and chitinolytic enzyme activities between desert and oasis flies of Phlebotomus papatasi to evaluate potential differences in susceptibility to infection with Leishmania major | Phlebotomus papatasi |
3.2.1.51 | analysis | comparison of glycolytic and chitinolytic enzyme activities between desert and oasis flies of Phlebotomus papatasi to evaluate potential differences in susceptibility to infection with Leishmania major | Phlebotomus papatasi |
3.2.1.52 | analysis | comparison of glycolytic and chitinolytic enzyme activities between desert and oasis flies of Phlebotomus papatasi to evaluate potential differences in susceptibility to infection with Leishmania major | Phlebotomus papatasi |
3.2.1.53 | analysis | comparison of glycolytic and chitinolytic enzyme activities between desert and oasis flies of Phlebotomus papatasi to evaluate potential differences in susceptibility to infection with Leishmania major | Phlebotomus papatasi |
3.2.1.88 | analysis | comparison of glycolytic and chitinolytic enzyme activities between desert and oasis flies of Phlebotomus papatasi to evaluate potential differences in susceptibility to infection with Leishmania major | Phlebotomus papatasi |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.1 | maltose | 86.2% relative inhibition, three concentrations of 10, 50, and 100 mM tested | Phlebotomus papatasi | |
3.2.1.14 | allosamidin | relative inhibition of 18.1%, six concentrations from 1 to 10 microM tested | Phlebotomus papatasi | |
3.2.1.14 | erythritol | relative inhibition of 18.1%, three concentrations of 10, 50, and 100 mM tested | Phlebotomus papatasi | |
3.2.1.14 | N-acetylgalactosamine | 76.2% relative inhibition, three concentrations of 10, 50, and 100 mM tested | Phlebotomus papatasi | |
3.2.1.14 | N-acetylglucosamine | 78.0% relative inhibition, three concentrations of 10, 50, and 100 mM tested | Phlebotomus papatasi | |
3.2.1.21 | erythritol | 73.3% relative inhibition | Phlebotomus papatasi | |
3.2.1.21 | glucose | 80.3% relative inhibition, efective inhibitor | Phlebotomus papatasi | |
3.2.1.21 | Inositol | 20% relative inhibition, poor inhibitor | Phlebotomus papatasi | |
3.2.1.21 | additional information | inhibiting saccharides used at three concentrations, 10, 50, and 100 mM | Phlebotomus papatasi | |
3.2.1.21 | sucrose | 36.4% relative inhibition | Phlebotomus papatasi | |
3.2.1.22 | galactose | relative inhibition of 83.8%, effective inhibitor | Phlebotomus papatasi | |
3.2.1.22 | additional information | inhibiting saccharides used at three concentrations, 10, 50, and 100 mM, no inhibitory effects of glucose, inositol, sucrose | Phlebotomus papatasi | |
3.2.1.24 | mannose | relative inhibition of 67.8%, potent inhibitor, three concentrations of 10, 50, and 100 mM tested | Phlebotomus papatasi | |
3.2.1.25 | mannose | relative inhibition of 20%, three concentrations of 10, 50, and 100 mM tested | Phlebotomus papatasi | |
3.2.1.52 | erythritol | 64.4% relative inhibition, three concentrations of 10, 50, and 100 mM tested | Phlebotomus papatasi | |
3.2.1.52 | N-acetylgalactosamine | 61.9% relative inhibition, three concentrations of 10, 50, and 100 mM tested | Phlebotomus papatasi | |
3.2.1.52 | N-acetylglucosamine | 76.2% relative inhibition, three concentrations of 10, 50, and 100 mM tested | Phlebotomus papatasi | |
3.2.1.53 | mannose | inhibition of beta-N-acetylgalactosaminidase but activation of alpha-N-acetylgalactosaminidase, three concentrations of 10, 50, and 100 mM tested | Phlebotomus papatasi | |
3.2.1.53 | N-acetylgalactosamine | dose dependent inhibition, at lower levels than inhibition by N-acetylglucosamine, three concentrations of 10, 50, and 100 mM tested | Phlebotomus papatasi | |
3.2.1.53 | N-acetylglucosamine | constant inhibition, three concentrations of 10, 50, and 100 mM tested | Phlebotomus papatasi |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.22 | sucrose + H2O | Phlebotomus papatasi | - |
alpha-D-glucose + D-fructose | - |
? | |
3.2.1.26 | sucrose + H2O | Phlebotomus papatasi | - |
alpha-D-glucose + D-fructose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.1 | Phlebotomus papatasi | - |
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid) | - |
3.2.1.14 | Phlebotomus papatasi | - |
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid) | - |
3.2.1.21 | Phlebotomus papatasi | - |
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid) | - |
3.2.1.22 | Phlebotomus papatasi | - |
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid) | - |
3.2.1.24 | Phlebotomus papatasi | - |
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid) | - |
3.2.1.25 | Phlebotomus papatasi | - |
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid) | - |
3.2.1.26 | Phlebotomus papatasi | - |
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid) | - |
3.2.1.37 | Phlebotomus papatasi | - |
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid) | - |
3.2.1.38 | Phlebotomus papatasi | - |
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid) | - |
3.2.1.50 | no acitvity in Phlebotomus papatasi | - |
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid) | - |
3.2.1.51 | Phlebotomus papatasi | - |
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid) | - |
3.2.1.52 | Phlebotomus papatasi | - |
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid) | - |
3.2.1.53 | Phlebotomus papatasi | - |
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid) | - |
3.2.1.88 | Phlebotomus papatasi | - |
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid) | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.1 | whole body | whole fly homogenates | Phlebotomus papatasi | - |
3.2.1.14 | whole body | whole fly homogenates | Phlebotomus papatasi | - |
3.2.1.21 | whole body | whole fly homogenates | Phlebotomus papatasi | - |
3.2.1.22 | whole body | whole fly homogenates | Phlebotomus papatasi | - |
3.2.1.24 | whole body | whole fly homogenates | Phlebotomus papatasi | - |
3.2.1.25 | whole body | whole fly homogenates | Phlebotomus papatasi | - |
3.2.1.26 | whole body | whole fly homogenates | Phlebotomus papatasi | - |
3.2.1.37 | whole body | whole fly homogenates | Phlebotomus papatasi | - |
3.2.1.38 | whole body | whole fly homogenates | Phlebotomus papatasi | - |
3.2.1.51 | whole body | whole fly homogenates | Phlebotomus papatasi | - |
3.2.1.52 | whole body | whole fly homogenates | Phlebotomus papatasi | - |
3.2.1.53 | whole body | whole fly homogenates | Phlebotomus papatasi | - |
3.2.1.88 | whole body | whole fly homogenates | Phlebotomus papatasi | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | additional information | - |
assay described, enzyme activity measured in homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity shown | Phlebotomus papatasi |
3.2.1.14 | additional information | - |
assay described, activity measured in whole body homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, no measurable activity by using the substrate 4-nitrophenyl-beta-N,N'-diacetylchitobioside, chitinase activity without any induction by blood- or sugar-meals observed | Phlebotomus papatasi |
3.2.1.21 | additional information | - |
assay described, enzyme activity measured in homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity shown, more activity produced by flies of the arid region than of those originating in sugar-rich environments, activity in desert females (Jordan Valley autumn line) decreased by 30.5% but increased in desert males by 14.7% | Phlebotomus papatasi |
3.2.1.22 | additional information | - |
assay described, enzyme activity measured in homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity shown, relative enzyme activities of whole fly homogenates low on substrates specific for alpha-galactosidase | Phlebotomus papatasi |
3.2.1.24 | additional information | - |
assay described, enzyme activity measured in homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity shown, enzyme activity of female colonies of the Jordan Valley autumn (arid) differs significantly from those of colonies of Neot Hakikar (oasis) and Kfar Adumim starved (arid) | Phlebotomus papatasi |
3.2.1.25 | additional information | - |
assay described, enzyme activity measured in homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity shown, reduced activity after feeding with sucrose observed | Phlebotomus papatasi |
3.2.1.26 | additional information | - |
assay described, enzyme activity measured in homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity shown, reduced activity after feeding with sucrose observed | Phlebotomus papatasi |
3.2.1.37 | additional information | - |
assay described, enzyme activity measured in homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity shown | Phlebotomus papatasi |
3.2.1.38 | additional information | - |
assay described, enzyme activity measured in homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity shown | Phlebotomus papatasi |
3.2.1.51 | additional information | - |
assay described, enzyme activity measured in homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity shown, flies of arid regions tend to produce more alpha-fucosidase activity than those originating in sugar-rich environments | Phlebotomus papatasi |
3.2.1.52 | additional information | - |
assay described, enzyme activity measured in homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity shown | Phlebotomus papatasi |
3.2.1.53 | additional information | - |
assay described, enzyme activity measured in homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity indicated | Phlebotomus papatasi |
3.2.1.88 | additional information | - |
assay described, activity measured in whole body homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity shown | Phlebotomus papatasi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.1 | 4-nitrophenyl-alpha-D-maltopentaoside + H2O | activity measured in total fly homogenates, substrate concentration of 2.2 mM in HEPES buffer, enzyme activity preferentially required for degrading starch components of plant tissue | Phlebotomus papatasi | 4-nitrophenol + alpha-D-maltopentaose | - |
? | |
3.2.1.14 | 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O | substrate concentration of 500 microM in citrate buffer | Phlebotomus papatasi | 4-methylumbelliferone + N,N'-diacetylchitobiose | - |
? | |
3.2.1.14 | additional information | 4-nitrophenyl-beta-N,N'-diacetylchitobioside shows no measurable activity in whole fly homogenates, substrate concentration of 22 mM in acetate phospate buffer used | Phlebotomus papatasi | ? | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | activity measured in total fly homogenates, substrate concentration of 60 mM in citrate buffer | Phlebotomus papatasi | 4-nitrophenol + D-glucopyranose | - |
? | |
3.2.1.22 | 4-nitrophenyl-alpha-D-galactopyranoside + H2O | activity measured in total fly homogenates, substrate concentration of 60 mM in citrate buffer | Phlebotomus papatasi | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
3.2.1.22 | sucrose + H2O | - |
Phlebotomus papatasi | alpha-D-glucose + D-fructose | - |
? | |
3.2.1.24 | 4-nitrophenyl-alpha-D-mannopyranoside + H2O | activity measured in whole fly homogenates, substrate concentration of 60 mM in citrate buffer, female oasis flies have 90% less alpha-mannosidase than females from the arid desert, flies of arid regions tend to produce more alpha-mannosidase activity than those originating in sugar-rich environments | Phlebotomus papatasi | 4-nitrophenol + alpha-D-mannopyranose | - |
? | |
3.2.1.25 | 4-nitrophenyl beta-D-mannopyranoside + H2O | activity measured in whole fly homogenates, substrate concentration of 6 mM in citrate buffer | Phlebotomus papatasi | 4-nitrophenol + beta-D-mannopyranose | - |
? | |
3.2.1.26 | sucrose + H2O | - |
Phlebotomus papatasi | alpha-D-glucose + D-fructose | - |
? | |
3.2.1.26 | sucrose + H2O | activity measured in whole fly homogenates, substrate concentration of 50 mM in sodium phosphate buffer | Phlebotomus papatasi | alpha-D-glucose + D-fructose | - |
? | |
3.2.1.37 | 4-nitrophenyl beta-D-xylopyranoside + H2O | low activity levels, measured in whole fly homogenates, substrate concentration of 60 mM in acetate phosphate buffer | Phlebotomus papatasi | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
3.2.1.38 | 4-nitrophenyl beta-D-fucoside + H2O | activity measured in whole fly homogenates, substrate concentration of 6 mM in citrate buffer | Phlebotomus papatasi | 4-nitrophenol + beta-D-fucose | - |
? | |
3.2.1.51 | 4-nitrophenyl-alpha-L-fucoside + H2O | activity measured in whole fly homogenates, substrate concentration of 6 mM in citrate buffer | Phlebotomus papatasi | 4-nitrophenol + alpha-L-fucose | - |
? | |
3.2.1.52 | 4-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O | activity measured in whole fly homogenates, substrate concentration of 6 mM in citrate buffer, female oasis flies produce more beta-N-acetylhexosaminidase than any other colony | Phlebotomus papatasi | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
3.2.1.53 | 4-nitrophenyl-N-acetyl-beta-D-galactosaminide + H2O | activity measured in whole fly homogenates, substrate concentration of 6 mM in citrate buffer, flies of arid regions tend to produce more beta-N-acetylgalactosaminidase than those originating in sugar-rich environments | Phlebotomus papatasi | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
3.2.1.88 | 4-nitrophenyl-alpha-arabinopyranoside + H2O | activity measured in whole fly homogenates, substrate concentration of 60 mM in acetate phospate buffer used | Phlebotomus papatasi | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.1 | alpha-amylase | - |
Phlebotomus papatasi |
3.2.1.14 | chitinase | - |
Phlebotomus papatasi |
3.2.1.21 | beta-glucosidase | - |
Phlebotomus papatasi |
3.2.1.22 | alpha-D-galactosidase | - |
Phlebotomus papatasi |
3.2.1.22 | alpha-galactosidase | - |
Phlebotomus papatasi |
3.2.1.24 | alpha-mannosidase | - |
Phlebotomus papatasi |
3.2.1.25 | beta-mannosidase | - |
Phlebotomus papatasi |
3.2.1.26 | beta-fructofuranosidase | - |
Phlebotomus papatasi |
3.2.1.37 | beta-xylosidase | - |
Phlebotomus papatasi |
3.2.1.37 | Xylan 1,4-beta-xylosidase | - |
Phlebotomus papatasi |
3.2.1.38 | beta-D-fucosidase | - |
Phlebotomus papatasi |
3.2.1.50 | alpha-N-acetylglucosaminidase | - |
no acitvity in Phlebotomus papatasi |
3.2.1.51 | alpha-L-fucosidase | - |
Phlebotomus papatasi |
3.2.1.52 | beta-N-acetylglucosaminidase | - |
Phlebotomus papatasi |
3.2.1.52 | Beta-N-acetylhexosaminidase | - |
Phlebotomus papatasi |
3.2.1.53 | beta-N-acetylgalactosaminidase | - |
Phlebotomus papatasi |
3.2.1.88 | beta-L-arabinosidase | - |
Phlebotomus papatasi |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 7.4 | - |
assay at, pH-optimum estimated for | Phlebotomus papatasi |
3.2.1.14 | 4.8 | - |
assay at, pH-optimum of 4.83 estimated for | Phlebotomus papatasi |
3.2.1.21 | 6.6 | - |
assay at, pH-optimum estimated for | Phlebotomus papatasi |
3.2.1.22 | 6.6 | - |
assay at, pH-optimum estimated for | Phlebotomus papatasi |
3.2.1.24 | 5.7 | - |
assay at, pH-optimum estimated for | Phlebotomus papatasi |
3.2.1.25 | 5.7 | - |
assay at, pH-optimum estimated for | Phlebotomus papatasi |
3.2.1.26 | 6 | - |
assay at, pH-optimum estimated for | Phlebotomus papatasi |
3.2.1.37 | 5.7 | - |
assay at, pH-optimum estimated for | Phlebotomus papatasi |
3.2.1.38 | 5.7 | - |
assay at, pH-optimum estimated for | Phlebotomus papatasi |
3.2.1.51 | 5.7 | - |
assay at, pH-optimum estimated for | Phlebotomus papatasi |
3.2.1.52 | 6.6 | - |
assay at, pH-optimum estimated for | Phlebotomus papatasi |
3.2.1.53 | 6.6 | - |
assay at, pH-optimum estimated for | Phlebotomus papatasi |
3.2.1.88 | 5.7 | - |
assay at, pH-optimum estimated for | Phlebotomus papatasi |