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Literature summary extracted from

  • Gargova, S.; Sariyska, M.; Angelov, A.; Stoilova, I.
    Aspergillus niger pH 2.1 optimum acid phosphatase with high affinity for phytate (2006), Folia Microbiol. (Praha), 51, 541-545.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.95
-
 4-nitrophenyl phosphate at pH 2.1 and 30°C Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Aspergillus niger
-
-
-
3.1.3.2 Aspergillus niger 307
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 Sephadex G-100 gel filtration, DEAE-Sepharose CL 6B column chromatography, and CM-Sepharose CL 6B column chromatography Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 9.66
-
 culture liquide Aspergillus niger
3.1.3.2 361.3
-
 after 37.5fold purification Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 1-naphthyl phosphate + H2O 163% activity compared to 4-nitrophenyl phosphate Aspergillus niger 1-naphthol + phosphate
-
 ?
3.1.3.2 1-naphthyl phosphate + H2O 163% activity compared to 4-nitrophenyl phosphate Aspergillus niger 307 1-naphthol + phosphate
-
 ?
3.1.3.2 2-phosphoenolpyruvate + H2O 75.6% activity compared to 4-nitrophenyl phosphate Aspergillus niger enolpyruvate + phosphate
-
 ?
3.1.3.2 2-phosphoglycerate + H2O 80.9% activity compared to 4-nitrophenyl phosphate Aspergillus niger glycerate + phosphate
-
 ?
3.1.3.2 2-phosphoglycerate + H2O 80.9% activity compared to 4-nitrophenyl phosphate Aspergillus niger 307 glycerate + phosphate
-
 ?
3.1.3.2 4-nitrophenyl phosphate + H2O 100% activity Aspergillus niger 4-nitrophenol + phosphate
-
 ?
3.1.3.2 4-nitrophenyl phosphate + H2O 100% activity Aspergillus niger 307 4-nitrophenol + phosphate
-
 ?
3.1.3.2 D-glucose 6-phosphate + H2O 85.2% activity compared to 4-nitrophenyl phosphate Aspergillus niger D-glucose + phosphate
-
 ?
3.1.3.2 D-glucose 6-phosphate + H2O 85.2% activity compared to 4-nitrophenyl phosphate Aspergillus niger 307 D-glucose + phosphate
-
 ?
3.1.3.2 dodecasodium phytate + H2O 245% activity compared to 4-nitrophenyl phosphate Aspergillus niger ? + phosphate
-
 ?
3.1.3.2 NADP+ + H2O 129% activity compared to 4-nitrophenyl phosphate Aspergillus niger NAD+ + phosphate
-
 ?
3.1.3.2 NADP+ + H2O 129% activity compared to 4-nitrophenyl phosphate Aspergillus niger 307 NAD+ + phosphate
-
 ?
3.1.3.2 phenylphosphate + H2O 134% activity compared to 4-nitrophenyl phosphate Aspergillus niger phenol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 pH 2.1 optimum acid phosphatase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 66
-
-
 Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 2.1
-
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.2 2 2.4
-
 Aspergillus niger