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Literature summary extracted from
- Biochemical and functional characterization of triosephosphate isomerase from Mycobacterium tuberculosis H37Rv (2006), FEMS Microbiol. Lett., 263, 229-235.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.1 | expression in Escherichia coli | Mycobacterium tuberculosis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.1 | 0.084 | - |
D-glyceraldehyde 3-phosphate | 22°C, pH 7.6 | Mycobacterium tuberculosis |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.1 | additional information | no metal cations are required | Mycobacterium tuberculosis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.1 | 28213 | - |
2 * 28213, mass spectrometry, 2 * 30000, SDS-PAGE | Mycobacterium tuberculosis |
| 5.3.1.1 | 30000 | - |
2 * 28213, mass spectrometry, 2 * 30000, SDS-PAGE | Mycobacterium tuberculosis |
| 5.3.1.1 | 60250 | - |
gel filtration | Mycobacterium tuberculosis |
| 5.3.1.1 | 66000 | - |
SDS-PAGE after UV cross-linking | Mycobacterium tuberculosis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.1 | Mycobacterium tuberculosis | - |
- |
- |
| 5.3.1.1 | Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.1.1 | recombinant enzyme | Mycobacterium tuberculosis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.1 | 5700 | - |
22°C, pH 7.6 | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.1 | D-glyceraldehyde 3-phosphate | - |
Mycobacterium tuberculosis | dihydroxyacetone phosphate | - |
? | |
| 5.3.1.1 | D-glyceraldehyde 3-phosphate | - |
Mycobacterium tuberculosis H37Rv | dihydroxyacetone phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.1.1 | dimer | 2 * 28213, mass spectrometry, 2 * 30000, SDS-PAGE | Mycobacterium tuberculosis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.1 | 37 | - |
- |
Mycobacterium tuberculosis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.1 | 50 | - |
stable up to | Mycobacterium tuberculosis |
| 5.3.1.1 | 60 | - |
30 min, complete inactivation | Mycobacterium tuberculosis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.1 | 68330 | - |
D-glyceraldehyde 3-phosphate | 22°C, pH 7.6 | Mycobacterium tuberculosis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.1 | 6 | 8 | - |
Mycobacterium tuberculosis |
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