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Literature summary extracted from

  • Moellering, E.R.; Ouyang, Y.; Mamedov, T.G.; Chollet, R.
    The two divergent PEP-carboxylase catalytic subunits in the green microalga Chlamydomonas reinhardtii respond reversibly to inorganic-N supply and co-exist in the high-molecular-mass, hetero-oligomeric Class-2 PEPC complex (2007), FEBS Lett., 581, 4871-4876.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.31 111000
-
 4 * 111000 Determined by: 1D native-PAGE and 2D BN-/SDS-PAGE, combined with immunoblotting Chlamydomonas reinhardtii
4.1.1.31 669000
-
 x * 669000, class-2 PEPC enzyme-forms a high-molecular-mass, hetero-oligomeric complex containing both CrPpc1 (p109) and CrPpc2 (p131) polypeptides. Determined by: 1D native–PAGE and 2D BN–/SDS–PAGE, combined with immunoblotting Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31 isoform-specific purification of CrPpc1 and CrPpc2 is carried out by affinity-purification of using CrPpc1 and CrPpc2 peptide antibodies Chlamydomonas reinhardtii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.31 leaf
-
 Chlamydomonas reinhardtii
-
4.1.1.31 additional information CrPpc1/2 polypeptide levels are up-regulated as the initial supply of NH4Cl decreases from 10 to 0.5 mM. However, within 5 h after re-supply of 10 mM NH4Cl to the N-deficient cells, the CrPpc1/2 levels reverts back nearly to those observed in high-N grown cells Chlamydomonas reinhardtii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.31 0.029
-
 with 7.5 mM NH4Cl Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 phosphoenolpyruvate + CO2
-
 Chlamydomonas reinhardtii phosphate + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.31 heteromer x * 669000, class-2 PEPC enzyme-forms a high-molecular-mass, hetero-oligomeric complex containing both CrPpc1 (p109) and CrPpc2 (p131) polypeptides. Determined by: 1D native–PAGE and 2D BN–/SDS–PAGE, combined with immunoblotting Chlamydomonas reinhardtii
4.1.1.31 homotetramer 4 * 111000 Determined by: 1D native-PAGE and 2D BN-/SDS-PAGE, combined with immunoblotting Chlamydomonas reinhardtii

Synonyms

EC Number Synonyms Comment Organism
4.1.1.31 Class-1 PEPC
-
 Chlamydomonas reinhardtii
4.1.1.31 Class-2 PEPC
-
 Chlamydomonas reinhardtii
4.1.1.31 CrPpc1
-
 Chlamydomonas reinhardtii
4.1.1.31 CrPpc2
-
 Chlamydomonas reinhardtii
4.1.1.31 PEP-carboxylase
-
 Chlamydomonas reinhardtii
4.1.1.31 PEPC
-
 Chlamydomonas reinhardtii
4.1.1.31 PPC
-
 Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.31 25
-
 assay at Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.31 8.4
-
 assay at Chlamydomonas reinhardtii