Literature summary extracted from

Stieglitz, K.A.; Roberts, M.F.; Li, W.; Stec, B.
Crystal structure of the tetrameric inositol 1-phosphate phosphatase (TM1415) from the hyperthermophile, Thermotoga maritima (2007), FEBS J., 274, 2461-2469.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.3.25	expressed in Escherichia coli	Thermotoga maritima

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.1.3.25	standing drop vapour diffusion method with 15% (w /v) PEG 4000, 15% (v /v) PEG 400, 0.2 M MgSO4, and 50 mM (NH4)2SO4, in 50 mM cacodylate buffer, pH 8	Thermotoga maritima

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.3.25	Thermotoga maritima	O33832	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.1.3.25	Sepharose Q FF column chromatography	Thermotoga maritima

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.3.25	glycerol 2-phosphate + H2O	-	Thermotoga maritima	glycerol + phosphate	-	?
3.1.3.25	L-myo-inositol 1-phosphate + H2O	-	Thermotoga maritima	L-myo-inositol + phosphate	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.1.3.25	tetramer	x-ray crystallography	Thermotoga maritima

Synonyms

EC Number	Synonyms	Comment	Organism
3.1.3.25	IMPase	-	Thermotoga maritima
3.1.3.25	inositol 1-phosphate phosphatase	-	Thermotoga maritima
3.1.3.25	TM1415	exhibits dual specificity with both IMPase and fructose-1,6-bisphosphatase activities	Thermotoga maritima

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)