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Literature summary extracted from

  • Carroll, J.D.; Pastuszak, I.; Edavana, V.K.; Pan, Y.T.; Elbein, A.D.
    A novel trehalase from Mycobacterium smegmatis - purification, properties, requirements (2007), FEBS J., 274, 1701-1714.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.28 alpha,alpha-trehalose the enzymatic activity increases with increasing concentrations of alpha,alpha-trehalose in the incubations up to about 50 mM Mycolicibacterium smegmatis
3.2.1.28 arsenate
-
 Mycolicibacterium smegmatis
3.2.1.28 phosphate
-
 Mycolicibacterium smegmatis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.28 expressed in Escherichia coli strain BL21 Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 AMP 50% inhibition at 20 mM Mycolicibacterium smegmatis
3.2.1.28 ATP 90% inhibition at 20 mM Mycolicibacterium smegmatis
3.2.1.28 castanospermine 50% inhibition at 0.5 mg/ml Mycolicibacterium smegmatis
3.2.1.28 diphosphate more than 50% inhibition at 8 mM and complete inhibition at 16 mM Mycolicibacterium smegmatis
3.2.1.28 additional information 40 mM sodium fluoride and trehazolin have no inhibitory effect Mycolicibacterium smegmatis
3.2.1.28 Polyphosphate
-
 Mycolicibacterium smegmatis
3.2.1.28 sodium orthovanadate 80% inhibition at 10 mM Mycolicibacterium smegmatis
3.2.1.28 UTP 90% inhibition at 10 mM Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 20
-
 alpha,alpha-trehalose at 37°C Mycolicibacterium smegmatis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.28 cytosol
-
 Mycolicibacterium smegmatis 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.28 Mg2+ absolutely required for activity, optimum activity occurring at concentrations of 3.5-4 mM Mycolicibacterium smegmatis
3.2.1.28 additional information Mg2+ can not be replaced by Ca2+, Mn2+ or Zn2+ Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 71000
-
 SDS-PAGE Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Mycolicibacterium smegmatis
-
-
-
3.2.1.28 Mycolicibacterium smegmatis b11
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28 ammonium sulfate fractionation, phenyl-Sepharose DE-52 column chromatography and Sephacryl S-300 gel filtration Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.28 0.227
-
 cell extract, at 37°C Mycolicibacterium smegmatis
3.2.1.28 36.99
-
 after 162fold purification, at 37°C Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O highly specific for alpha,alpha-trehalose Mycolicibacterium smegmatis 2 D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O highly specific for alpha,alpha-trehalose Mycolicibacterium smegmatis b11 2 D-glucose
-
 ?
3.2.1.28 additional information does not hydrolyze alpha,beta-trehalose, beta,beta-trehalose, trehalose dimycolate, or any other alpha-glucoside or beta-glucoside Mycolicibacterium smegmatis ?
-
 ?
3.2.1.28 additional information does not hydrolyze alpha,beta-trehalose, beta,beta-trehalose, trehalose dimycolate, or any other alpha-glucoside or beta-glucoside Mycolicibacterium smegmatis b11 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 multimer 20 or more subunits, gel filtration Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.28 MSMEG 4528
-
 Mycolicibacterium smegmatis
3.2.1.28 trehalase
-
 Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 7.1
-
-
 Mycolicibacterium smegmatis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.28 6.5 8 the trehalase activity drops sharply at pH values of 6.5 and below, as well as at pH values of 8.0 and above Mycolicibacterium smegmatis