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Literature summary extracted from

  • Cao, Y.; Yang, P.; Shi, P.; Wang, Y.; Luo, H.; Meng, K.; Zhang, Z.; Wu, N.; Yao, B.; Fan, Y.
    Purification and characterization of a novel protease-resistant alpha-galactosidase from Rhizopus sp. F78 ACCC 30795 (2007), Enzyme Microb. Technol., 41, 835-841.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 Ag+ no activity at 1 mM Rhizopus sp.
3.2.1.22 Al3+ 66.7% activity at 1 mM Rhizopus sp.
3.2.1.22 Ca2+ 82.2% activity at 1 mM Rhizopus sp.
3.2.1.22 Co2+ 96.6% activity at 1 mM Rhizopus sp.
3.2.1.22 Cr3+ 63.9% activity at 1 mM Rhizopus sp.
3.2.1.22 Cu2+ 78.1% activity at 1 mM Rhizopus sp.
3.2.1.22 EDTA 98.1% activity at 1 mM Rhizopus sp.
3.2.1.22 Fe2+ 75.1% activity at 1 mM Rhizopus sp.
3.2.1.22 Hg2+ no activity at 1 mM Rhizopus sp.
3.2.1.22 Li+ 96.7% activity at 1 mM Rhizopus sp.
3.2.1.22 Mg2+ 98.2% activity at 1 mM Rhizopus sp.
3.2.1.22 Mn2+ 49.5% activity at 1 mM Rhizopus sp.
3.2.1.22 additional information not inhibited by Na+ and Zn2+ Rhizopus sp.
3.2.1.22 Ni(2+) 92.7% activity at 1 mM Rhizopus sp.
3.2.1.22 SDS no activity at 1 mM Rhizopus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 2.9
-
p-nitrophenyl alpha-D-galactopyranoside in 0.1 M McIlvaine buffer (pH 4.8) at 37°C Rhizopus sp.
3.2.1.22 33
-
melibiose in 0.1 M McIlvaine buffer (pH 4.8) at 37°C Rhizopus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.22 K+ 9.5% increased activity at 1 mM Rhizopus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 78000
-
SDS-PAGE Rhizopus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Rhizopus sp.
-
18S rRNA sequence; ACCC 30795
-
3.2.1.22 Rhizopus sp. F78
-
18S rRNA sequence; ACCC 30795
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22 ammonium sulfate precipitation, HiTrap Q Sepharose XL column chromatography, and Sephacryl S-200 HR gel filtration Rhizopus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 1.2
-
crude filtrate Rhizopus sp.
3.2.1.22 74.6
-
after 60.4fold purification Rhizopus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 melibiose + H2O
-
Rhizopus sp. D-galactose + D-glucose
-
?
3.2.1.22 melibiose + H2O
-
Rhizopus sp. F78 D-galactose + D-glucose
-
?
3.2.1.22 additional information no detectable activity towards guar gum Rhizopus sp. ?
-
?
3.2.1.22 additional information no detectable activity towards guar gum Rhizopus sp. F78 ?
-
?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Rhizopus sp. p-nitrophenol + alpha-D-galactose
-
?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Rhizopus sp. F78 p-nitrophenol + alpha-D-galactose
-
?
3.2.1.22 raffinose + H2O
-
Rhizopus sp. D-galactose + sucrose
-
?
3.2.1.22 raffinose + H2O
-
Rhizopus sp. F78 D-galactose + sucrose
-
?
3.2.1.22 stachyose + 2 H2O
-
Rhizopus sp. 2 alpha-D-galactose + sucrose
-
?
3.2.1.22 stachyose + 2 H2O
-
Rhizopus sp. F78 2 alpha-D-galactose + sucrose
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 Aga-F78
-
Rhizopus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 50
-
-
Rhizopus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 40 60 activity is 80% of maximum between 40°C and 55°C, the enzyme retains less than 25% of its activity at above 60°C and is stable at 50°C, no activity is lost after 30 min incubation, after 10, 20 and 30 min incubation at 60°C, 38%, 18% and 7% of activity remains, respectively Rhizopus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 4.8
-
-
Rhizopus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.22 3 10 activity is not detected when the enzyme is reacted in buffers under pH 3.0 or above pH 10.0, activity remains above 80% between pH 4.6-5.2, the enzyme retains more than 80% of its activity between pH 5.0 and 10.0 Rhizopus sp.