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Literature summary extracted from

  • Wang, Y.; Gao, X.; Su, Q.; Wu, W.; An, L.
    Cloning, expression, and enzyme characterization of an acid heat-stable phytase from Aspergillus fumigatus WY-2 (2007), Curr. Microbiol., 55, 65-70.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 expressed in Pichia pastoris strain GS115 Aspergillus fumigatus

General Stability

EC Number General Stability Organism
3.1.3.8 incubated with pepsin at 37°C for 2 h at the ratio (pepsin/phytase, wt/wt) of 0.1, the enzyme still retains 90.1% residual activity Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 Al3+ 1 mM, inhibitive effect Aspergillus fumigatus
3.1.3.8 Cu2+ 1 mM, inhibitive effect Aspergillus fumigatus
3.1.3.8 Fe3+ 1 mM, inhibitive effect Aspergillus fumigatus
3.1.3.8 Zn2+ 1 mM, inhibitive effect Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.114
-
 myo-inositol hexakisphosphate in 200 mM sodium acetate buffer (pH 5.5), at 37°C Aspergillus fumigatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.8 Ca2+ 1 mM, slight stimulatory effect Aspergillus fumigatus
3.1.3.8 Co2+ 1 mM, slight stimulatory effect Aspergillus fumigatus
3.1.3.8 K+ 1 mM, slight stimulatory effect Aspergillus fumigatus
3.1.3.8 Mg2+ 1 mM, slight stimulatory effect Aspergillus fumigatus
3.1.3.8 Mn2+ 1 mM, slight stimulatory effect Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 88000
-
 SDS-PAGE Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus fumigatus Q5XNQ9 3-phytase A precursor
-
3.1.3.8 Aspergillus fumigatus WY-2 Q5XNQ9 3-phytase A precursor
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 Q-DEAE-Sepharose column chromatography Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Aspergillus fumigatus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Aspergillus fumigatus WY-2 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 myo-inositol hexakiphosphate phosphohydrolase
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 55
-
-
 Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 90
-
 high ability to resist heat inactivation, after being exposed to 90°C for 15 min, the recombinant enzyme in crude and purified form retains 42% and 43.7% of the initial enzyme activity, respectively Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.8 102
-
 myo-inositol hexakisphosphate
-
 Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5.5
-
-
 Aspergillus fumigatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.8 2
-
 very stable at the low pH, losing no activity when incubated at pH 2.0 for 4 h Aspergillus fumigatus
3.1.3.8 7
-
 displays a sharp decline in the activity as the pH moves toward the neutral range, and maintains negligible activity beyond pH 7.0 Aspergillus fumigatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.8 Aspergillus fumigatus theoretical value
-
 5.95