EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.7 | A460I | strongly decreased activity | Pseudomonas putida |
4.1.1.7 | A460I/F464I | strongly decreased activity | Pseudomonas putida |
4.1.1.7 | F464I | strongly decreased activity | Pseudomonas putida |
4.1.1.7 | H281A | strongly decreased activity | Pseudomonas putida |
4.1.1.7 | H70A | strongly decreased activity | Pseudomonas putida |
4.1.1.7 | S26A | strongly decreased activity | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.7 | 0.2 | - |
benzoylformate | mutant enzyme A460I | Pseudomonas putida | |
4.1.1.7 | 0.4 | - |
benzoylformate | mutant enzyme H281A | Pseudomonas putida | |
4.1.1.7 | 0.8 | - |
benzoylformate | wild type enzyme | Pseudomonas putida | |
4.1.1.7 | 1.1 | - |
benzoylformate | mutant enzyme A460I/F464I | Pseudomonas putida | |
4.1.1.7 | 1.9 | - |
benzoylformate | mutant enzyme H70A | Pseudomonas putida | |
4.1.1.7 | 2.3 | - |
benzoylformate | mutant enzyme F464I | Pseudomonas putida | |
4.1.1.7 | 4.5 | - |
benzoylformate | mutant enzyme S26A | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.7 | Pseudomonas putida | P20906 | - |
- |
4.1.2.38 | Pseudomonas fluorescens | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.7 | benzaldehyde + benzaldehyde | - |
Pseudomonas putida | (R)-benzoin | - |
? | |
4.1.1.7 | benzoylformate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? | |
4.1.1.7 | Benzoylformate + acetaldehyde | - |
Pseudomonas putida | (S)-2-Hydroxypropiophenone | - |
? | |
4.1.1.7 | additional information | does not perform the cleavage of 2-hydroxy ketones | Pseudomonas putida | ? | - |
? | |
4.1.2.38 | 2-hydroxypropiophenone | - |
Pseudomonas fluorescens | ? | - |
? | |
4.1.2.38 | benzoin | - |
Pseudomonas fluorescens | benzaldehyde + benzaldehyde | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.7 | tetramer | - |
Pseudomonas putida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.7 | BFD | catalyzes a carboligase reaction that yields 2-hydroxypropiophenone, by using benzoylformate and acetaldehyde as substrates, and is able to catalyze the carboligation of a broad range of aldehydes | Pseudomonas putida |
4.1.2.38 | BAL | - |
Pseudomonas fluorescens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.7 | thiamine diphosphate | dependent | Pseudomonas putida | |
4.1.2.38 | thiamine diphosphate | dependent | Pseudomonas fluorescens |