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Literature summary extracted from

  • Chen, S.; Galan, M.C.; Coltharp, C.; OConnor, S.E.
    Redesign of a central enzyme in alkaloid biosynthesis (2006), Chem. Biol., 13, 1137-1141.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.3.3.2 biotechnology redesigning the substrate specificity of strictosidine synthase is an important step toward re-engineering the TIA pathway to produce products with novel or improved biological properties Catharanthus roseus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.2 for expression in Escherichia coli BL21DE3 cells Catharanthus roseus

Protein Variants

EC Number Protein Variants Comment Organism
4.3.3.2 D177A mutant engineered for redesigning the substrate specificity Catharanthus roseus
4.3.3.2 E277G mutant engineered for redesigning the substrate specificity Catharanthus roseus
4.3.3.2 F232T mutant engineered for redesigning the substrate specificity Catharanthus roseus
4.3.3.2 F314A mutant engineered for redesigning the substrate specificity Catharanthus roseus
4.3.3.2 F330L mutant engineered for redesigning the substrate specificity Catharanthus roseus
4.3.3.2 H283A mutant engineered for redesigning the substrate specificity Catharanthus roseus
4.3.3.2 H313N mutant engineered for redesigning the substrate specificity Catharanthus roseus
4.3.3.2 L329G mutant engineered for redesigning the substrate specificity Catharanthus roseus
4.3.3.2 Q282A mutant engineered for redesigning the substrate specificity Catharanthus roseus
4.3.3.2 S275G mutant engineered for redesigning the substrate specificity Catharanthus roseus
4.3.3.2 V182G mutant engineered for redesigning the substrate specificity Catharanthus roseus
4.3.3.2 W153F mutant engineered for redesigning the substrate specificity Catharanthus roseus
4.3.3.2 Y109H mutant engineered for redesigning the substrate specificity Catharanthus roseus
4.3.3.2 Y155A mutant engineered for redesigning the substrate specificity Catharanthus roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.3.2 3-alpha(S)-strictosidine + H2O Catharanthus roseus
-
tryptamine + secologanin
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.2 Catharanthus roseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.2 by one-step affinity purification using a amylose column Catharanthus roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.2 3-alpha(S)-strictosidine + H2O
-
Catharanthus roseus tryptamine + secologanin
-
?

Synonyms

EC Number Synonyms Comment Organism
4.3.3.2 str
-
Catharanthus roseus
4.3.3.2 strictosidine synthase
-
Catharanthus roseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.3.2 30
-
HPLC activity assay Catharanthus roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.3.2 6.8
-
HPLC activity assay Catharanthus roseus