EC Number | Application | Comment | Organism |
---|---|---|---|
4.3.3.2 | biotechnology | redesigning the substrate specificity of strictosidine synthase is an important step toward re-engineering the TIA pathway to produce products with novel or improved biological properties | Catharanthus roseus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.3.3.2 | for expression in Escherichia coli BL21DE3 cells | Catharanthus roseus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.3.3.2 | D177A | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
4.3.3.2 | E277G | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
4.3.3.2 | F232T | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
4.3.3.2 | F314A | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
4.3.3.2 | F330L | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
4.3.3.2 | H283A | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
4.3.3.2 | H313N | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
4.3.3.2 | L329G | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
4.3.3.2 | Q282A | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
4.3.3.2 | S275G | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
4.3.3.2 | V182G | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
4.3.3.2 | W153F | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
4.3.3.2 | Y109H | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
4.3.3.2 | Y155A | mutant engineered for redesigning the substrate specificity | Catharanthus roseus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.3.2 | 3-alpha(S)-strictosidine + H2O | Catharanthus roseus | - |
tryptamine + secologanin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.3.2 | Catharanthus roseus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.3.2 | by one-step affinity purification using a amylose column | Catharanthus roseus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.3.2 | 3-alpha(S)-strictosidine + H2O | - |
Catharanthus roseus | tryptamine + secologanin | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.3.3.2 | str | - |
Catharanthus roseus |
4.3.3.2 | strictosidine synthase | - |
Catharanthus roseus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.3.3.2 | 30 | - |
HPLC activity assay | Catharanthus roseus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.3.3.2 | 6.8 | - |
HPLC activity assay | Catharanthus roseus |