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Literature summary extracted from

  • Okamoto, R.; Kato, T.; Mizoguchi, A.; Takahashi, N.; Nakakuki, T.; Mizutani, H.; Isaka, N.; Imanaka-Yoshida, K.; Kaibuchi, K.; Lu, Z.; Mabuchi, K.; Tao, T.; Hartshorne, D.J.; Nakano, T.; Ito, M.
    Characterization and function of MYPT2, a target subunit of myosin phosphatase in heart (2006), Cell. Signal., 18, 1408-1416.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.53 overexpression of MYPT1 and MYPT2 in primary cultures of cardiac myocytes and in COS7 cells using baculoviral and adenoviral expression systems Mammalia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.53 0.00103
-
 myosin light-chain phosphate smooth muscle, type 1 protein phosphatase delta and MYPT1 Mammalia
3.1.3.53 0.00136
-
 myosin light-chain phosphate smooth muscle, type 1 protein phosphatase delta and MYPT2 Mammalia
3.1.3.53 0.00394
-
 myosin light-chain phosphate smooth muscle, type 1 protein phosphatase delta only Mammalia
3.1.3.53 0.00502
-
 myosin light-chain phosphate cardiac muscle, type 1 protein phosphatase delta and MYPT1 Mammalia
3.1.3.53 0.00551
-
 myosin light-chain phosphate cardiac muscle, type 1 protein phosphatase delta and MYPT2 Mammalia
3.1.3.53 0.01117
-
 myosin light-chain phosphate cardiac muscle, type 1 protein phosphatase delta only Mammalia

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.53 additional information close to the Z-line, the A-Band and mitochondrion Mammalia
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.53 130000
-
 Western blot analysis Mammalia

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.53 Mammalia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.53 of the native and recombinant proteins by immunoprecipitation Mammalia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.53 cardiac muscle
-
 Mammalia
-
3.1.3.53 smooth muscle
-
 Mammalia
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.53 myosin light-chain phosphate + H2O
-
 Mammalia myosin light-chain + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.53 additional information enzyme has 3 subunits, the catalytic subunit type 1 protein phosphatase delta, the regulatory subunit MYPT1 in smooth muscle and MYPT 2 an isoform of MYPT1 in striated muscle Mammalia

Synonyms

EC Number Synonyms Comment Organism
3.1.3.53 MP
-
 Mammalia
3.1.3.53 myosin phosphatase
-
 Mammalia
3.1.3.53 [myosin-light-chain] phosphatase
-
 Mammalia