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Literature summary extracted from

  • Gong, P.F.; Xu, J.H.; Tang, Y.F.; Wu, H.Y.
    Improved catalytic performance of Bacillus megaterium epoxide hydrolase in a medium containing Tween-80 (2003), Biotechnol. Prog., 19, 652-654.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.3.2.10 Tween-80 when the cosolvent DMSO is replaced by an emulsifier (Tween-80, 0.5% w/v) as an alternative additive to help disperse the water-insoluble substrate, the apparent activity of the epoxide hydrolase significantly increased by about 1.8fold Priestia megaterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.3.2.10 acetone
-
 Priestia megaterium
3.3.2.10 Ag+ 5 mM, more than 90% inhibition Priestia megaterium
3.3.2.10 Al3+ 5 mM, more than 90% inhibition Priestia megaterium
3.3.2.10 Cu2+ 5 mM, more than 90% inhibition Priestia megaterium
3.3.2.10 Fe2+ 5 mM, more than 90% inhibition Priestia megaterium
3.3.2.10 Fe3+ 5 mM, more than 90% inhibition Priestia megaterium
3.3.2.10 Ni2+ 5 mM, more than 90% inhibition Priestia megaterium
3.3.2.10 tetrahydrofuran
-
 Priestia megaterium
3.3.2.10 Zn2+ 5 mM, more than 90% inhibition Priestia megaterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.3.2.10 Ca2+ 5 mM, activity is enhanced to 108% of control Priestia megaterium
3.3.2.10 Mg2+ 5 mM, activity is enhanced to 112% of control Priestia megaterium
3.3.2.10 Mn2+ 5 mM, activity is enhanced to 110% of control Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.10 Priestia megaterium
-
-
-
3.3.2.10 Priestia megaterium ECU1001
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.3.2.10 partial Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.10 (R)-glycidyl phenyl ether + H2O the hydrolysis of (R)-epoxide in the presence of Tween-80 is 25.6 times faster than that of the (S)-epoxide Priestia megaterium ?
-
 ?
3.3.2.10 (R)-glycidyl phenyl ether + H2O the hydrolysis of (R)-epoxide in the presence of Tween-80 is 25.6 times faster than that of the (S)-epoxide Priestia megaterium ECU1001 ?
-
 ?
3.3.2.10 2-methylphenyl glycidyl ether + H2O
-
 Priestia megaterium ?
-
 ?
3.3.2.10 2-methylphenyl glycidyl ether + H2O
-
 Priestia megaterium ECU1001 ?
-
 ?
3.3.2.10 4-methylphenylglycidyl ether + H2O
-
 Priestia megaterium ?
-
 ?
3.3.2.10 4-methylphenylglycidyl ether + H2O
-
 Priestia megaterium ECU1001 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.3.2.10 30
-
-
 Priestia megaterium
3.3.2.10 40
-
 when the cosolvent DMSO is replaced by an emulsifier (Tween-80, 0.5% w/v) as an alternative additive to help disperse the water-insoluble substrate the temperature optimum shifts from 30°C to 40°C Priestia megaterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.3.2.10 30
-
 1 h, no loss of activity Priestia megaterium
3.3.2.10 40
-
 6 h, stable Priestia megaterium
3.3.2.10 50
-
 when the cosolvent DMSO is replaced by an emulsifier (Tween-80, 0.5% w/v) as an alternative additive to help disperse the water-insoluble substrate the half-life of the enzyme at 50°C increases by 2.5times Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.3.2.10 6.5
-
-
 Priestia megaterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.3.2.10 6.5 7.5 quite stable at Priestia megaterium