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Literature summary extracted from
- Cloning, expression and characterization of a Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440 (2007), Biotechnol. Lett., 29, 1393-1398.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.13.92 | BVMO gene, functional expression in Escherichia coli strains JM109 and BL21(DE3) as soluble protein | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.92 | 2-decanone + NADPH + O2 | Pseudomonas putida | - |
? + NADP+ + H2O | - |
? |  |
| 1.14.13.92 | 2-decanone + NADPH + O2 | Pseudomonas putida KT 2240 | - |
? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 2-dodecanone + NADPH + O2 | Pseudomonas putida | - |
? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 2-nonanone + NADPH + O2 | Pseudomonas putida | - |
? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 2-nonanone + NADPH + O2 | Pseudomonas putida KT 2240 | - |
? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 2-undecanone + NADPH + O2 | Pseudomonas putida | - |
? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 2-undecanone + NADPH + O2 | Pseudomonas putida KT 2240 | - |
? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 3-decanone + NADPH + O2 | Pseudomonas putida | - |
? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 3-decanone + NADPH + O2 | Pseudomonas putida KT 2240 | - |
? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 4-decanone + NADPH + O2 | Pseudomonas putida | - |
? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 4-decanone + NADPH + O2 | Pseudomonas putida KT 2240 | - |
? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | additional information | Pseudomonas putida | substrate specificity and reaction mechanism, the enzyme shows high specificity towards short-chain aliphatic ketones, some open-chain ketones are converted to the alkylacetates, while for others formation of the ester products with oxygen on the other side of the keto group can also be detected yielding the corresponding methyl or ethyl esters, overview | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.92 | Pseudomonas putida | - |
BVMO gene | - |
| 1.14.13.92 | Pseudomonas putida KT 2240 | - |
BVMO gene | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.92 | 2-decanone + NADPH + O2 | - |
Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 2-decanone + NADPH + O2 | - |
Pseudomonas putida KT 2240 | ? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 2-dodecanone + NADPH + O2 | - |
Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 2-nonanone + NADPH + O2 | - |
Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 2-nonanone + NADPH + O2 | - |
Pseudomonas putida KT 2240 | ? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 2-undecanone + NADPH + O2 | - |
Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 2-undecanone + NADPH + O2 | - |
Pseudomonas putida KT 2240 | ? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 3-decanone + NADPH + O2 | - |
Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 3-decanone + NADPH + O2 | - |
Pseudomonas putida KT 2240 | ? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 4-decanone + NADPH + O2 | - |
Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 4-decanone + NADPH + O2 | best substrate | Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | 4-decanone + NADPH + O2 | - |
Pseudomonas putida KT 2240 | ? + NADP+ + H2O | - |
? | |
| 1.14.13.92 | additional information | substrate specificity and reaction mechanism, the enzyme shows high specificity towards short-chain aliphatic ketones, some open-chain ketones are converted to the alkylacetates, while for others formation of the ester products with oxygen on the other side of the keto group can also be detected yielding the corresponding methyl or ethyl esters, overview | Pseudomonas putida | ? | - |
? | |
| 1.14.13.92 | additional information | the enzyme shows high specificity towards short-chain aliphatic ketones, some open-chain ketones are converted to the alkylacetates, while for others formation of the ester products with oxygen on the other side of the keto group can also be detected yielding the corresponding methyl or ethyl esters, overview, no activity with 2-octanone and 2-tridecanone | Pseudomonas putida | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.92 | Baeyer-Villiger monooxygenase | - |
Pseudomonas putida |
| 1.14.13.92 | BVMO | - |
Pseudomonas putida |
| 1.14.13.92 | additional information | the enzyme belongs to the Baeyer-Villiger monooxygenases | Pseudomonas putida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.92 | 20 | 30 | biotransformation assay | Pseudomonas putida |
| 1.14.13.92 | 25 | - |
assay at | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.92 | 7.5 | - |
assay at | Pseudomonas putida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.92 | NADPH | - |
Pseudomonas putida | |
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