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Literature summary extracted from

  • Rehdorf, J.; Kirschner, A.; Bornscheuer, U.T.
    Cloning, expression and characterization of a Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440 (2007), Biotechnol. Lett., 29, 1393-1398.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.92 BVMO gene, functional expression in Escherichia coli strains JM109 and BL21(DE3) as soluble protein Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.92 2-decanone + NADPH + O2 Pseudomonas putida
-
 ? + NADP+ + H2O
-
 ?
1.14.13.92 2-decanone + NADPH + O2 Pseudomonas putida KT 2240
-
 ? + NADP+ + H2O
-
 ?
1.14.13.92 2-dodecanone + NADPH + O2 Pseudomonas putida
-
 ? + NADP+ + H2O
-
 ?
1.14.13.92 2-dodecanone + NADPH + O2 Pseudomonas putida KT 2240
-
 ? + NADP+ + H2O
-
 ?
1.14.13.92 2-nonanone + NADPH + O2 Pseudomonas putida
-
 ? + NADP+ + H2O
-
 ?
1.14.13.92 2-nonanone + NADPH + O2 Pseudomonas putida KT 2240
-
 ? + NADP+ + H2O
-
 ?
1.14.13.92 2-undecanone + NADPH + O2 Pseudomonas putida
-
 ? + NADP+ + H2O
-
 ?
1.14.13.92 3-decanone + NADPH + O2 Pseudomonas putida
-
 ? + NADP+ + H2O
-
 ?
1.14.13.92 4-decanone + NADPH + O2 Pseudomonas putida
-
 ? + NADP+ + H2O
-
 ?
1.14.13.92 additional information Pseudomonas putida substrate specificity and reaction mechanism, the enzyme shows high specificity towards short-chain aliphatic ketones, some open-chain ketones are converted to the alkylacetates, while for others formation of the ester products with oxygen on the other side of the keto group can also be detected yielding the corresponding methyl or ethyl esters, overview ?
-
 ?
1.14.13.92 additional information Pseudomonas putida KT 2240 substrate specificity and reaction mechanism, the enzyme shows high specificity towards short-chain aliphatic ketones, some open-chain ketones are converted to the alkylacetates, while for others formation of the ester products with oxygen on the other side of the keto group can also be detected yielding the corresponding methyl or ethyl esters, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.92 Pseudomonas putida
-
 BVMO gene
-
1.14.13.92 Pseudomonas putida KT 2240
-
 BVMO gene
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.92 2-decanone + NADPH + O2
-
 Pseudomonas putida ? + NADP+ + H2O
-
 ?
1.14.13.92 2-decanone + NADPH + O2
-
 Pseudomonas putida KT 2240 ? + NADP+ + H2O
-
 ?
1.14.13.92 2-dodecanone + NADPH + O2
-
 Pseudomonas putida ? + NADP+ + H2O
-
 ?
1.14.13.92 2-dodecanone + NADPH + O2
-
 Pseudomonas putida KT 2240 ? + NADP+ + H2O
-
 ?
1.14.13.92 2-nonanone + NADPH + O2
-
 Pseudomonas putida ? + NADP+ + H2O
-
 ?
1.14.13.92 2-nonanone + NADPH + O2
-
 Pseudomonas putida KT 2240 ? + NADP+ + H2O
-
 ?
1.14.13.92 2-undecanone + NADPH + O2
-
 Pseudomonas putida ? + NADP+ + H2O
-
 ?
1.14.13.92 3-decanone + NADPH + O2
-
 Pseudomonas putida ? + NADP+ + H2O
-
 ?
1.14.13.92 4-decanone + NADPH + O2
-
 Pseudomonas putida ? + NADP+ + H2O
-
 ?
1.14.13.92 4-decanone + NADPH + O2 best substrate Pseudomonas putida ? + NADP+ + H2O
-
 ?
1.14.13.92 additional information substrate specificity and reaction mechanism, the enzyme shows high specificity towards short-chain aliphatic ketones, some open-chain ketones are converted to the alkylacetates, while for others formation of the ester products with oxygen on the other side of the keto group can also be detected yielding the corresponding methyl or ethyl esters, overview Pseudomonas putida ?
-
 ?
1.14.13.92 additional information the enzyme shows high specificity towards short-chain aliphatic ketones, some open-chain ketones are converted to the alkylacetates, while for others formation of the ester products with oxygen on the other side of the keto group can also be detected yielding the corresponding methyl or ethyl esters, overview, no activity with 2-octanone and 2-tridecanone Pseudomonas putida ?
-
 ?
1.14.13.92 additional information substrate specificity and reaction mechanism, the enzyme shows high specificity towards short-chain aliphatic ketones, some open-chain ketones are converted to the alkylacetates, while for others formation of the ester products with oxygen on the other side of the keto group can also be detected yielding the corresponding methyl or ethyl esters, overview Pseudomonas putida KT 2240 ?
-
 ?
1.14.13.92 additional information the enzyme shows high specificity towards short-chain aliphatic ketones, some open-chain ketones are converted to the alkylacetates, while for others formation of the ester products with oxygen on the other side of the keto group can also be detected yielding the corresponding methyl or ethyl esters, overview, no activity with 2-octanone and 2-tridecanone Pseudomonas putida KT 2240 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.92 Baeyer-Villiger monooxygenase
-
 Pseudomonas putida
1.14.13.92 BVMO
-
 Pseudomonas putida
1.14.13.92 More the enzyme belongs to the Baeyer-Villiger monooxygenases Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.92 20 30 biotransformation assay Pseudomonas putida
1.14.13.92 25
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.92 7.5
-
 assay at Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.92 NADPH
-
 Pseudomonas putida