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Literature summary extracted from

  • Fernandez-Lorente, G.; Palomo, J.M.; Cabrera, Z.; Fernandez-Lafuente, R.; Guisan, J.M.
    Improved catalytic properties of immobilized lipases by the presence of very low concentrations of detergents in the reaction medium (2007), Biotechnol. Bioeng., 97, 242-250.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 CTAB PFL immobilized on glyoxyl and BrCN agarose is activates 8fold at 0.01% CTAB Pseudomonas fluorescens
3.1.1.3 additional information detergents mediate hyperactivation, effect of the detergents is to shift the conformational equilibrium of lipases toward the open form Pseudomonas fluorescens
3.1.1.3 additional information detergents mediate hyperactivation, effect of the detergents is to shift the conformational equilibrium of lipases toward the open form Moesziomyces antarcticus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.3 additional information immobilization of the enzyme on glyoxyl-agarose at pH 10.0 and 25°C and on BrCN agarose, overview Pseudomonas fluorescens
3.1.1.3 additional information immobilization of the enzyme on glyoxyl-agarose at pH 10.0 and 25°C and on BrCN agarose, overview Moesziomyces antarcticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride the presence of detergents, e.g. SDS, CTAB, Triton X-100, or Triton X-45, permitts the inhibition of lipase by irreversible covalent inhibitors, e.g. 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride, AEBSF, while the enzyme, in the absence of detergent, is not inhibited by these irreversible inhibitors Moesziomyces antarcticus
3.1.1.3 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride the presence of detergents, e.g. SDS, CTAB, Triton X-100, or Triton X-45, permits the inhibition of lipase by irreversible covalent inhibitors, e.g. 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride, AEBSF, while the enzyme, in the absence of detergent, is not inhibited by these irreversible inhibitors Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Moesziomyces antarcticus
-
 isozyme b
-
3.1.1.3 Pseudomonas fluorescens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 commercial preparation
-
 Pseudomonas fluorescens
-
3.1.1.3 commercial preparation
-
 Moesziomyces antarcticus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 additional information
-
 activities of the enzyme immobilized on glyoxyl-agarose and on BrCN agarose, overview Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 (+-)-2-hydroxy-4-phenylbutyric acid ethyl ester + H2O enantioselectivity of the immobilized enzyme in this reaction is increased 40fold by presence of 0.1% CTAB Pseudomonas fluorescens ?
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Pseudomonas fluorescens 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Moesziomyces antarcticus 4-nitrophenol + palmitate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 PFL
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 25
-
 assay at Pseudomonas fluorescens
3.1.1.3 25
-
 assay at Moesziomyces antarcticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 7
-
 assay at Pseudomonas fluorescens
3.1.1.3 7
-
 assay at Moesziomyces antarcticus