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Literature summary extracted from

  • Walker, A.W.; Keasling, J.D.
    Metabolic engineering of Pseudomonas putida for the utilization of parathion as a carbon and energy source. (2002), Biotechnol. Bioeng., 78, 715-721.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.8.1 gene opd, expression of the gene with and without secretory leader sequence using the tac and taclac promoters Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
3.1.8.1 additional information transformation of Pseudomnas putida with the opd gene and the 4-nitrophenyl PNP operon allows the engineered organism to utilize parathion as sole source of carbon and energy, overview Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.1.8.1 Pseudomonas putida
-
 gene opd
-
3.1.8.1 Pseudomonas putida KT 2442
-
 gene opd
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.8.1 cell culture an engineered strain growing on parathion Pseudomonas putida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.8.1 additional information
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.8.1 parathion + H2O engneered mutant strain, parathion metabolism, overview Pseudomonas putida 4-nitrophenol + diethyl thiophosphate
-
 ?
3.1.8.1 parathion + H2O engneered mutant strain, parathion metabolism, overview Pseudomonas putida KT 2442 4-nitrophenol + diethyl thiophosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.8.1 OPH
-
 Pseudomonas putida
3.1.8.1 organophosphate hydrolase
-
 Pseudomonas putida