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Literature summary extracted from

  • Li, S.; Kim, W.D.; Kaneko, S.; Prema, P.A.; Nakajima, M.; Kobayashi, H.
    Expression of rice (Oryza sativa L. var. Nipponbare) alpha-galactosidase genes in Escherichia coli and characterization (2007), Biosci. Biotechnol. Biochem., 71, 520-526.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.22 expressed in Escherichia coli strain Origami (DE) Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 Ag+ alpha-Gal II, 67% decrease of activity at 1 mM; alpha-Gal III, 84% decrease of activity at 1 mM Oryza sativa
3.2.1.22 Hg2+ alpha-Gal II, 93% decrease of activity at 1 mM; alpha-Gal III, 87% decrease of activity at 1 mM Oryza sativa
3.2.1.22 p-chloromercuribenzoate alpha-Gal III shows less than 8% of the original activity by treatment with 0.1 mM p-chloromercuribenzoate at 20°C for 1 h; alpha-Gal II shows less than 6% of the original activity by treatment with 0.1 mM p-chloromercuribenzoate at 20°C for 1 h Oryza sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.22 additional information Ca2+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+, and Zn2+ do not affect activity Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 41020
-
calculated from amino acid sequence Oryza sativa
3.2.1.22 41520
-
calculated from amino acid sequence Oryza sativa
3.2.1.22 42000
-
SDS-PAGE Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Oryza sativa Q7XIV4 variant Nipponbare
-
3.2.1.22 Oryza sativa Q9FXT4 alpha-Gal II; variant Nipponbare
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22 Ni2+-Sepharose column chromatography Oryza sativa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 0.31
-
alpha-Gal III, crude extract Oryza sativa
3.2.1.22 2.25
-
alpha-Gal II, crude extract Oryza sativa
3.2.1.22 9.13
-
alpha-Gal III, after 29.5fold purification Oryza sativa
3.2.1.22 52.8
-
alpha-Gal II, after 23.5fold purification Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 6(1)-alpha-D-galactosyl mannobiose + H2O 2.6% relative activity compared to p-nitrophenyl alpha-D-galactoside Oryza sativa ?
-
?
3.2.1.22 6(1)-alpha-D-galactosyl mannobiose + H2O 6.8% relative activity compared to p-nitrophenyl alpha-D-galactoside Oryza sativa ?
-
?
3.2.1.22 6(1)-alpha-D-galactosyl mannotriose + H2O 2.7% relative activity compared to p-nitrophenyl alpha-D-galactoside Oryza sativa ?
-
?
3.2.1.22 6(1)-alpha-D-galactosyl mannotriose + H2O 6.9% relative activity compared to p-nitrophenyl alpha-D-galactoside Oryza sativa ?
-
?
3.2.1.22 6(3),6(4)-di-alpha-D-galactosyl mannopentaose + H2O 0.5% relative activity compared to p-nitrophenyl alpha-D-galactoside Oryza sativa ?
-
?
3.2.1.22 6(3),6(4)-di-alpha-D-galactosyl mannopentaose + H2O 7.3% relative activity compared to p-nitrophenyl alpha-D-galactoside Oryza sativa ?
-
?
3.2.1.22 guar gum + H2O 0.4% relative activity compared to p-nitrophenyl alpha-D-galactoside Oryza sativa ?
-
?
3.2.1.22 guar gum + H2O 1.7% relative activity compared to p-nitrophenyl alpha-D-galactoside Oryza sativa ?
-
?
3.2.1.22 locust bean gum + H2O 0.4% relative activity compared to p-nitrophenyl alpha-D-galactoside Oryza sativa ?
-
?
3.2.1.22 locust bean gum + H2O 1.4% relative activity compared to p-nitrophenyl alpha-D-galactoside Oryza sativa ?
-
?
3.2.1.22 melibiose + H2O 1.2% relative activity compared to p-nitrophenyl alpha-D-galactoside Oryza sativa D-galactose + D-glucose
-
?
3.2.1.22 melibiose + H2O 6.9% relative activity compared to p-nitrophenyl alpha-D-galactoside Oryza sativa D-galactose + D-glucose
-
?
3.2.1.22 p-nitrophenyl alpha-D-galactoside + H2O 100% activity Oryza sativa p-nitrophenol + alpha-D-galactose
-
?
3.2.1.22 raffinose + H2O 1.89% relative activity compared to p-nitrophenyl alpha-D-galactoside Oryza sativa sucrose + alpha-D-galactose
-
?
3.2.1.22 raffinose + H2O 10.1% relative activity compared to p-nitrophenyl alpha-D-galactoside Oryza sativa sucrose + alpha-D-galactose
-
?
3.2.1.22 stachyose + 2 H2O 0.47% relative activity compared to p-nitrophenyl alpha-D-galactoside Oryza sativa 2 alpha-D-galactose + sucrose
-
?
3.2.1.22 stachyose + 2 H2O 1.9% relative activity compared to p-nitrophenyl alpha-D-galactoside Oryza sativa 2 alpha-D-galactose + sucrose
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 alpha-Gal II
-
Oryza sativa
3.2.1.22 alpha-Gal III
-
Oryza sativa
3.2.1.22 alpha-galactosidase II
-
Oryza sativa
3.2.1.22 alpha-galactosidase III
-
Oryza sativa
3.2.1.22 alpha-galactoside galactohydrolase
-
Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 45
-
-
Oryza sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 35
-
alpha-Gal II is stable for 1 h up to 35°C at pH 5.0 Oryza sativa
3.2.1.22 35
-
alpha-Gal III is stable for 1 h up to 20°C at pH 5.0 Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 5
-
-
Oryza sativa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.22 3.5 8.5 alpha-Gal II is stable at pH 3.5-8.5 at 25°C Oryza sativa
3.2.1.22 4 7 alpha-Gal III is stable at pH 4.0-7.0 at 20°C Oryza sativa