EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.22 | expressed in Escherichia coli strain Origami (DE) | Oryza sativa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.22 | Ag+ | alpha-Gal II, 67% decrease of activity at 1 mM; alpha-Gal III, 84% decrease of activity at 1 mM | Oryza sativa | |
3.2.1.22 | Hg2+ | alpha-Gal II, 93% decrease of activity at 1 mM; alpha-Gal III, 87% decrease of activity at 1 mM | Oryza sativa | |
3.2.1.22 | p-chloromercuribenzoate | alpha-Gal III shows less than 8% of the original activity by treatment with 0.1 mM p-chloromercuribenzoate at 20°C for 1 h; alpha-Gal II shows less than 6% of the original activity by treatment with 0.1 mM p-chloromercuribenzoate at 20°C for 1 h | Oryza sativa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.22 | additional information | Ca2+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+, and Zn2+ do not affect activity | Oryza sativa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 41020 | - |
calculated from amino acid sequence | Oryza sativa |
3.2.1.22 | 41520 | - |
calculated from amino acid sequence | Oryza sativa |
3.2.1.22 | 42000 | - |
SDS-PAGE | Oryza sativa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.22 | Oryza sativa | Q7XIV4 | variant Nipponbare | - |
3.2.1.22 | Oryza sativa | Q9FXT4 | alpha-Gal II; variant Nipponbare | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.22 | Ni2+-Sepharose column chromatography | Oryza sativa |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 0.31 | - |
alpha-Gal III, crude extract | Oryza sativa |
3.2.1.22 | 2.25 | - |
alpha-Gal II, crude extract | Oryza sativa |
3.2.1.22 | 9.13 | - |
alpha-Gal III, after 29.5fold purification | Oryza sativa |
3.2.1.22 | 52.8 | - |
alpha-Gal II, after 23.5fold purification | Oryza sativa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.22 | 6(1)-alpha-D-galactosyl mannobiose + H2O | 2.6% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
3.2.1.22 | 6(1)-alpha-D-galactosyl mannobiose + H2O | 6.8% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
3.2.1.22 | 6(1)-alpha-D-galactosyl mannotriose + H2O | 2.7% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
3.2.1.22 | 6(1)-alpha-D-galactosyl mannotriose + H2O | 6.9% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
3.2.1.22 | 6(3),6(4)-di-alpha-D-galactosyl mannopentaose + H2O | 0.5% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
3.2.1.22 | 6(3),6(4)-di-alpha-D-galactosyl mannopentaose + H2O | 7.3% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
3.2.1.22 | guar gum + H2O | 0.4% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
3.2.1.22 | guar gum + H2O | 1.7% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
3.2.1.22 | locust bean gum + H2O | 0.4% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
3.2.1.22 | locust bean gum + H2O | 1.4% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
3.2.1.22 | melibiose + H2O | 1.2% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | D-galactose + D-glucose | - |
? | |
3.2.1.22 | melibiose + H2O | 6.9% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | D-galactose + D-glucose | - |
? | |
3.2.1.22 | p-nitrophenyl alpha-D-galactoside + H2O | 100% activity | Oryza sativa | p-nitrophenol + alpha-D-galactose | - |
? | |
3.2.1.22 | raffinose + H2O | 1.89% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | sucrose + alpha-D-galactose | - |
? | |
3.2.1.22 | raffinose + H2O | 10.1% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | sucrose + alpha-D-galactose | - |
? | |
3.2.1.22 | stachyose + 2 H2O | 0.47% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | 2 alpha-D-galactose + sucrose | - |
? | |
3.2.1.22 | stachyose + 2 H2O | 1.9% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | 2 alpha-D-galactose + sucrose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.22 | alpha-Gal II | - |
Oryza sativa |
3.2.1.22 | alpha-Gal III | - |
Oryza sativa |
3.2.1.22 | alpha-galactosidase II | - |
Oryza sativa |
3.2.1.22 | alpha-galactosidase III | - |
Oryza sativa |
3.2.1.22 | alpha-galactoside galactohydrolase | - |
Oryza sativa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 45 | - |
- |
Oryza sativa |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 35 | - |
alpha-Gal II is stable for 1 h up to 35°C at pH 5.0 | Oryza sativa |
3.2.1.22 | 35 | - |
alpha-Gal III is stable for 1 h up to 20°C at pH 5.0 | Oryza sativa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 5 | - |
- |
Oryza sativa |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 3.5 | 8.5 | alpha-Gal II is stable at pH 3.5-8.5 at 25°C | Oryza sativa |
3.2.1.22 | 4 | 7 | alpha-Gal III is stable at pH 4.0-7.0 at 20°C | Oryza sativa |