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Literature summary extracted from

  • Watanabe, J.; Tanaka, H.; Akagawa, T.; Mogi, Y.; Yamazaki, T.
    Characterization of Aspergillus oryzae aspartyl aminopeptidase expressed in Escherichia coli (2007), Biosci. Biotechnol. Biochem., 71, 2557-2560.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.21 expressed in Escherichia coli Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.21 Ca2+ 57% residual activity at 40 mM; 57% residual activity at 40 mM Aspergillus oryzae
3.4.11.21 EDTA 54% residual activity at 20 mM; 54% residual activity at 20 mM Aspergillus oryzae
3.4.11.21 NaCl about 30% of activity is retained in 20% (w/v) NaCl; about 30% of activity is retained in 20% (w/v) NaCl Aspergillus oryzae
3.4.11.21 o-phenanthroline 43% residual activity at 5 mM; 43% residual activity at 5 mM Aspergillus oryzae
3.4.11.21 Zn2+ 27% residual activity at 0.4 mM; 27% residual activity at 0.4 mM Aspergillus oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.21 Co2+ 294% increase of activity at 0.4 mM Aspergillus oryzae
3.4.11.21 Mn2+ 91% increase of activity at 0.4 mM Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.21 54430
-
 calculated from amino acid sequence Aspergillus oryzae
3.4.11.21 57700
-
 SDS-PAGE Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.21 Aspergillus oryzae Q2U9Z9
-
-
3.4.11.21 Aspergillus oryzae Q2UPZ7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.21 Ni-Sepharose 6 Fast Flow column chromatography Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.21 angiotensin II + H2O
-
 Aspergillus oryzae Asp + angiotensin III
-
 ?
3.4.11.21 Asp-p-nitroanilide + H2O 64% activity Aspergillus oryzae Asp + p-nitroaniline
-
 ?
3.4.11.21 Glu-p-nitroanilide + H2O 100% activity Aspergillus oryzae Glu + p-nitroaniline
-
 ?
3.4.11.21 additional information does not hydrolyze [Asn1, Val5]-angiotensin II, less than 0.5% activity on Ala-p-nitroanilide, Pro-p-nitroanilide, Met-p-nitroanilide, Arg-p-nitroanilide, and Leu-p-nitroanilide Aspergillus oryzae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.21 angiotensinase
-
 Aspergillus oryzae
3.4.11.21 AspAP
-
 Aspergillus oryzae
3.4.11.21 aspartyl-AP
-
 Aspergillus oryzae
3.4.11.21 DAP
-
 Aspergillus oryzae
3.4.11.21 peptidase E
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.21 50
-
-
 Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.21 50
-
 the enzyme remains stabel upt to 50°C and retains about 70% activity after 20 h at 50°C, is inactivated rapidly at temperature in excess of this Aspergillus oryzae
3.4.11.21 50
-
 the enzyme remains stable upt to 50°C and retains about 70% activity after 20 h at 50°C, is inactivated rapidly at temperature in excess of this Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.21 7
-
-
 Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.21 6 9
-
 Aspergillus oryzae