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Literature summary extracted from

  • Li, Z.; Wang, Z.; Peng, G.; Yin, Y.; Zhao, H.; Cao, Y.; Xia, Y.
    Purification and characterization of a novel thermostable extracellular protein tyrosine phosphatase from Metarhizium anisopliae strain CQMa102 (2006), Biosci. Biotechnol. Biochem., 70, 1961-1968.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.48 beta-mercaptoethanol 6% increase of activity at 20 mM Metarhizium anisopliae
3.1.3.48 dithiothreitol 7.4% increase of activity at 20 mM Metarhizium anisopliae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.48 Ag+ 16.6% inhibition at 2 mM Metarhizium anisopliae
3.1.3.48 Cu2+ 32.2% inhibition at 2 mM Metarhizium anisopliae
3.1.3.48 Fe2+ 34.5% inhibition at 2 mM Metarhizium anisopliae
3.1.3.48 molybdate 92.2% inhibition at 5 mM Metarhizium anisopliae
3.1.3.48 additional information EDTA, sodium tartrate, sodium fluoride, or okadaic acid have no effect on activity Metarhizium anisopliae
3.1.3.48 N-Methylmaleimide 76.3% inhibition at 0.5 mM Metarhizium anisopliae
3.1.3.48 orthovanadate 24.5% inhibition at 5 mM Metarhizium anisopliae
3.1.3.48 tungstate 16.3% inhibition at 20 mM Metarhizium anisopliae
3.1.3.48 Zn2+ 75.2% inhibition at 5 mM Metarhizium anisopliae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.48 Ba2+ 42.5% increase of activity at 20 mM Metarhizium anisopliae
3.1.3.48 Mn2+ 9.2% increase of activity at 20 mM Metarhizium anisopliae
3.1.3.48 additional information Mg2+, Co2+, or Ca2+ have no effect on activity Metarhizium anisopliae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.48 82500
-
 SDS-PAGE Metarhizium anisopliae

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.48 Metarhizium anisopliae
-
-
-
3.1.3.48 Metarhizium anisopliae CQMa102
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.48 ConA-Sepharose 4B column chromatography, HighQ anion resin chromatography, and 25S cation resin column chromatography Metarhizium anisopliae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.48 0.045
-
 culture medium, using p-nitrophenyl phosphate as a substrate Metarhizium anisopliae
3.1.3.48 8.681
-
 after 192.9fold purification, using p-nitrophenyl phosphate as a substrate Metarhizium anisopliae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.48 ATP + H2O 9.5% activity compared to TRDIpYETDYYRK Metarhizium anisopliae ADP + phosphate
-
 ?
3.1.3.48 ATP + H2O 9.5% activity compared to TRDIpYETDYYRK Metarhizium anisopliae CQMa102 ADP + phosphate
-
 ?
3.1.3.48 additional information no activity against phytic acid, ADP, AMP, O-phospho-L-serine or O-phospho-L-threonine Metarhizium anisopliae ?
-
 ?
3.1.3.48 additional information no activity against phytic acid, ADP, AMP, O-phospho-L-serine or O-phospho-L-threonine Metarhizium anisopliae CQMa102 ?
-
 ?
3.1.3.48 O-phospho-L-tyrosine + H2O
-
 Metarhizium anisopliae L-tyrosine + phosphate
-
 ?
3.1.3.48 O-phospho-L-tyrosine + H2O
-
 Metarhizium anisopliae CQMa102 L-tyrosine + phosphate
-
 ?
3.1.3.48 p-nitrophenyl phosphate + H2O 60.3% activity compared to TRDIpYETDYYRK Metarhizium anisopliae p-nitrophenol + phosphate
-
 ?
3.1.3.48 p-nitrophenyl phosphate + H2O 60.3% activity compared to TRDIpYETDYYRK Metarhizium anisopliae CQMa102 p-nitrophenol + phosphate
-
 ?
3.1.3.48 TRDIpYETDYYRK + H2O 100% activity Metarhizium anisopliae TRDIYETDYYRK + phosphate
-
 ?
3.1.3.48 TRDIpYETDYYRK + H2O 100% activity Metarhizium anisopliae CQMa102 TRDIYETDYYRK + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.48 protein tyrosine phosphatase
-
 Metarhizium anisopliae
3.1.3.48 PTPase
-
 Metarhizium anisopliae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.48 75
-
-
 Metarhizium anisopliae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.48 30 70 remains stable after 1 h at up to 70°C Metarhizium anisopliae
3.1.3.48 80
-
 loses 31% activity after 10 min at 80°C Metarhizium anisopliae
3.1.3.48 90
-
 complete loss of activity after 10 min at 90°C Metarhizium anisopliae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.48 5.5
-
-
 Metarhizium anisopliae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.48 3 9.5 at 30°C Metarhizium anisopliae
3.1.3.48 4.5 8 stable at 70°C, but activity decreases markedly at pH below 4.5 or pH above 8.0 Metarhizium anisopliae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.48 Metarhizium anisopliae isoelectric focusing
-
 9.5