Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sharma, S.; Agarwal, L.; Saxena, R.K.
    Purification, immobilization and characterization of tannase from Penicillium variable (2007), Biores. Technol., 99, 2544-2551.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.20 sodium cholate
-
 Talaromyces variabilis
3.1.1.20 sodium taurocholate
-
 Talaromyces variabilis

Application

EC Number Application Comment Organism
3.1.1.20 synthesis the enzyme immobilized on Amberlite IR retains about 85% of the initial catalytic activity even after ninth cycle of its use, immobilization method optimization, overview Talaromyces variabilis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.20 additional information the enzyme immobilized on Amberlite IR retains about 85% of the initial catalytic activity even after ninth cycle of its use, immobilization method optimization, overview Talaromyces variabilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.20 1,10-phenanthroline
-
 Talaromyces variabilis
3.1.1.20 1-[(naphthalen-1-yl)oxy]-3-[(propan-2-yl)amino]propan-2-ol
-
 Talaromyces variabilis
3.1.1.20 acetone complete inactivation Talaromyces variabilis
3.1.1.20 beta-mercaptoethanol
-
 Talaromyces variabilis
3.1.1.20 Bromoacetic acid
-
 Talaromyces variabilis
3.1.1.20 carbon tetrachloride complete inactivation Talaromyces variabilis
3.1.1.20 cyanamide
-
 Talaromyces variabilis
3.1.1.20 DTT
-
 Talaromyces variabilis
3.1.1.20 ethanol
-
 Talaromyces variabilis
3.1.1.20 formaldehyde complete inactivation Talaromyces variabilis
3.1.1.20 heptane
-
 Talaromyces variabilis
3.1.1.20 iodoacetic acid
-
 Talaromyces variabilis
3.1.1.20 mercuric benzoic acid
-
 Talaromyces variabilis
3.1.1.20 additional information no inhibition by Triton X-100 Talaromyces variabilis
3.1.1.20 NEM 81% inhibition Talaromyces variabilis
3.1.1.20 petroleum ether
-
 Talaromyces variabilis
3.1.1.20 phenyl boronic acid
-
 Talaromyces variabilis
3.1.1.20 PMSF 72% inhibition Talaromyces variabilis
3.1.1.20 SDS complete inactivation Talaromyces variabilis
3.1.1.20 sodium cholate
-
 Talaromyces variabilis
3.1.1.20 tetrahydrofuran complete inactivation Talaromyces variabilis
3.1.1.20 Toluene
-
 Talaromyces variabilis
3.1.1.20 Tween-60 complete inactivation Talaromyces variabilis
3.1.1.20 Tween-80 complete inactivation Talaromyces variabilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.20 32
-
 Tannic acid pH 5.0, 50°C Talaromyces variabilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.20 158000
-
 2 * 158000, SDS-PAGE Talaromyces variabilis
3.1.1.20 310000
-
 gel filtration Talaromyces variabilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.20 tannic acid + H2O Talaromyces variabilis
-
 gallate + ?
-
 ?
3.1.1.20 tannic acid + H2O Talaromyces variabilis IARI 2031
-
 gallate + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Talaromyces variabilis
-
-
-
3.1.1.20 Talaromyces variabilis IARI 2031
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 native enzyme 135fold by ultrafiltration using 100 kDa molecular weight cut off and gel filtration Talaromyces variabilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.20 2055
-
 purified native enzyme Talaromyces variabilis

Storage Stability

EC Number Storage Stability Organism
3.1.1.20 shelf-life of crude and crude lyophilized tannase at 4°C and at 30°C, 70% remaining activity after 365 days, overview Talaromyces variabilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 tannic acid + H2O
-
 Talaromyces variabilis gallate + ?
-
 ?
3.1.1.20 tannic acid + H2O tannins from Chebulina myrobalan Talaromyces variabilis gallate + ?
-
 ?
3.1.1.20 tannic acid + H2O
-
 Talaromyces variabilis IARI 2031 gallate + ?
-
 ?
3.1.1.20 tannic acid + H2O tannins from Chebulina myrobalan Talaromyces variabilis IARI 2031 gallate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.20 dimer 2 * 158000, SDS-PAGE Talaromyces variabilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 50
-
-
 Talaromyces variabilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.20 25 80 activity range Talaromyces variabilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 5
-
-
 Talaromyces variabilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.20 3 8 activity range Talaromyces variabilis