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Literature summary extracted from

  • Sukdeo, N.; Honek, J.F.
    Pseudomonas aeruginosa contains multiple glyoxalase I-encoding genes from both metal activation classes (2007), Biochim. Biophys. Acta, 1774, 756-763.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.4.1.5 additional information Pseudomonas aeruginosa possesses GlxI enzymes from two metal activation classes. The gloA1 and gloA2 genes encode non-Zn2+ dependent glyoxalase I enzymes and the gloA3 gene encodes a Zn2+-dependent homolog Pseudomonas aeruginosa PAO1

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.5 gloA2 and gloA3 ligated into vector pET22b, overexpression in Escherichia coli BL21(gammaDE3) Pseudomonas aeruginosa PAO1

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.5 1,10-phenanthroline decreases GloA3 activity Pseudomonas aeruginosa PAO1
4.4.1.5 dipicolinic acid greatest loss of GloA3 activity Pseudomonas aeruginosa PAO1

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.5 Cd2+ reactivates GloA3 by 87% after treatment with dipicolinic acid Pseudomonas aeruginosa PAO1
4.4.1.5 Co2+ increases GloA2 activity, hyper-reactivates GloA3 by 115% after treatment with dipicolinic acid Pseudomonas aeruginosa PAO1
4.4.1.5 Mg2+ reactivates GloA3 by 26% after treatment with dipicolinic acid Pseudomonas aeruginosa PAO1
4.4.1.5 Mn2+ hyper-reactivates GloA3 by 146% after treatment with dipicolinic acid Pseudomonas aeruginosa PAO1
4.4.1.5 Ni2+ increases GloA2 activity, hyper-reactivates GloA3 by 146% after treatment with dipicolinic acid Pseudomonas aeruginosa PAO1
4.4.1.5 Zn2+ does not increase GloA2 activity, GloA3 contains Zn2+, reactivates GloA3 by 76% after treatment with dipicolinic acid Pseudomonas aeruginosa PAO1

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.5 Pseudomonas aeruginosa PAO1 Q9HU72
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.5 GloA2 and GloA3, by gel filtration Pseudomonas aeruginosa PAO1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.5 glutathione + methylglyoxal
-
 Pseudomonas aeruginosa PAO1 S-D-lactoylglutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.5 dimer GloA2 and GloA3, gel filtration Pseudomonas aeruginosa PAO1

Synonyms

EC Number Synonyms Comment Organism
4.4.1.5 GloA1
-
 Pseudomonas aeruginosa PAO1
4.4.1.5 GloA2
-
 Pseudomonas aeruginosa PAO1
4.4.1.5 GloA3
-
 Pseudomonas aeruginosa PAO1
4.4.1.5 GLXI
-
 Pseudomonas aeruginosa PAO1
4.4.1.5 glyoxalase I
-
 Pseudomonas aeruginosa PAO1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.5 6.6
-
-
 Pseudomonas aeruginosa PAO1