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Literature summary extracted from

  • Morishige, J.; Touchika, K.; Tanaka, T.; Satouchi, K.; Fukuzawa, K.; Tokumura, A.
    Production of bioactive lysophosphatidic acid by lysophospholipase D in hen egg white (2007), Biochim. Biophys. Acta, 1771, 491-499.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.39 1,10-phenanthroline inhibitory effects of 1,10-phenanthroline at different concentrations on lysoPLD activity against palmitoyl-lysophosphatidylcholine Gallus gallus
3.1.4.39 bovine serum albumin concentration-dependent inhibition on lysoPLD activity in egg white during 24 h incubation Gallus gallus
3.1.4.39 EDTA inhibitory effects of EDTA at different concentrations on lysoPLD activity against palmitoyl-lysophosphatidylcholine. Inhibitory effect disappears when Mn2+ or Co2+ are added with EDTA to the egg white. Co-addition of Zn2+, Ni2+, or Cu2+ with EDTA results in 2-3times higher lysoPLD activity Gallus gallus
3.1.4.39 lysozyme concentration-dependent inhibition on lysoPLD activity in egg white during 24 h incubation Gallus gallus
3.1.4.39 additional information Hypothesis that a protein in hen egg white masks the lysoPLD activity in undiluted egg white, and that dilution at more than 10times results in a high decline of the inhibitory activity Gallus gallus
3.1.4.39 palmitoyl-lysophosphatidic acid concentration-dependent inhibition of lysoPLD activity in egg white during 24 h incubation. More than 100 microM of lysophosphatidic acid needed to inhibit significantly lysoPLD activity of hen egg white Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.39 extracellular
-
 Gallus gallus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.39 Co2+ lysoPLD activity is increased when Co2+ is added. Addition of Zn2+, Mn2+, Ni2+, or Co2+ to diluted egg white alters preference patterns of lysoPLD toward choline-containing substrates Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.39 110000
-
 Western blot analysis of AXT/lysoPLD Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.39 lysophosphatidylcholine + H2O Gallus gallus
-
 lysophosphatidic acid + choline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.39 Gallus gallus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.39 egg white lysoPLD secreted in several animal body fluids. Hen egg white originates mainly from the hen oviduct fluid, which contains various bioactive molecules acting on the oviduct, ova, and sperm Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.39 additional information
-
 lysoPLD activity of diluted or undiluted egg white measured based on enzyme-linked fluorometric measurement of choline produced together with lysophosphatidic acid from exogenous lysophosphatidylcholine. LysoPLD activity measured by determining lysophosphatidic acid and choline by MALDI-TOF-MS, mass spectrometric and enzyme-linked fluorometric analysis. Quantitative analysis of lysophosphatidic acid by MALDI-TOF-MS using a phosphate-capture molecule. Production of lysophosphatidic acid during incubation of hen egg white, changes in the levels of fatty acyl groups Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.39 lysophosphatidylcholine + H2O
-
 Gallus gallus lysophosphatidic acid + choline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.39 lysophospholipase D
-
 Gallus gallus
3.1.4.39 lysoPLD
-
 Gallus gallus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.39 37
-
 assay at Gallus gallus