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Literature summary extracted from

  • Islas-Flores, I.; Villanueva, M.A.
    Inositol-1 (or 4)-monophosphatase from Glycine max embryo axes is a phosphatase with broad substrate specificity that includes phytate dephosphorylation (2007), Biochim. Biophys. Acta, 1770, 543-550.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.25 Li+
-
 Glycine max
3.1.3.25 additional information not inhibited by butanedione monoxime, or orthovanadate Glycine max
3.1.3.25 NaF
-
 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.25 0.235
-
 diphosphate at 37°C, in 25 mM phosphate buffer with 2 mM dithiothreitol (pH 7.0) and 2 mM MgCl2 Glycine max

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.25 Ca2+ activates Glycine max
3.1.3.25 Co2+ activates Glycine max
3.1.3.25 Cu2+ activates Glycine max
3.1.3.25 Mg2+ activates Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.25 29000
-
 SDS-PAGE Glycine max

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.25 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.25 Sephacryl S-300 gel filtration, hydroxyapatite column chromatography, and DEAE-Sephacel column chromatography Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.25 embryo
-
 Glycine max
-
3.1.3.25 hypocotyl
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.25 4.37
-
 total cell extract, at 37°C, using sodium diphosphate supplemented with MgCl2 as a substrate Glycine max
3.1.3.25 375.6
-
 after 85.94fold purification, at 37°C, using sodium diphosphate supplemented with MgCl2 as a substrate Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.25 ATP + H2O
-
 Glycine max ADP + phosphate
-
 ?
3.1.3.25 D-myo-inositol 1,2,3,4,5,6-hexakisphosphate + H2O
-
 Glycine max D-myo-inositol 1,2,3,4,5-pentakisphosphate + phosphate
-
 ?
3.1.3.25 D-myo-inositol 1-phosphate + H2O
-
 Glycine max D-myo-inositol + phosphate
-
 ?
3.1.3.25 diphosphate + H2O
-
 Glycine max 2 phosphate
-
 ?
3.1.3.25 additional information does not hydrolyze p-nitrophenyl phosphate, ADP, AMP or D-glucose 6-phosphate Glycine max ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.25 IMPase
-
 Glycine max
3.1.3.25 inositol-1(or 4)-monophosphatase
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.25 37
-
-
 Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.25 37 80
-
 Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.25 7.4
-
-
 Glycine max

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.25 Glycine max isoelectric focusing
-
 5.9