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Literature summary extracted from
- Exploring the specific features of interfacial enzymology based on lipase studies (2006), Biochim. Biophys. Acta, 1761, 995-1013.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.3 | Lipid | the enzyme requires lipids for activity, complete separation of bound lipids from the enzyme leads to extensive loss of activity | Yarrowia lipolytica |  |
| 3.1.1.3 | sodium taurodeoxycholate | activates at 2 mM | Yarrowia lipolytica | |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | medicine | the enzyme can be used in enzyme replacement therapy treating pancreatic exocrine insufficiency | Yarrowia lipolytica |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.3 | enzyme overexpression from genetically modified gene lip2 multicopy strain under control of POX2 promoter | Yarrowia lipolytica |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | additional information | construction of strains containing multicopies of gene lip2 | Yarrowia lipolytica |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.3 | additional information | no inhibition of YLLIP2 by bile salts at micellar concentrations | Yarrowia lipolytica |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 33385 | - |
x * 38000, SDS-PAGE, x * 36874-38481, four glycosylated isozymes, mass spectrometry, x * 33385, nonglycosylated enzyme, mass spectrometry | Yarrowia lipolytica |
| 3.1.1.3 | 38000 | - |
x * 38000, SDS-PAGE, x * 36874-38481, four glycosylated isozymes, mass spectrometry, x * 33385, nonglycosylated enzyme, mass spectrometry | Yarrowia lipolytica |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.3 | Yarrowia lipolytica | Q9P8F7 | four LIP2 isozymes | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | glycoprotein | four isozymes with different glycosylation patterns, overview | Yarrowia lipolytica |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.3 | LIP2 from engineered strains with lip2 multicopy gene by gel filtration and cation exchange chromatography | Yarrowia lipolytica |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 2200 | - |
purified YLLIP2, substrate soybean oil | Yarrowia lipolytica |
| 3.1.1.3 | 10760 | - |
purified YLLIP2, substrate tributyrin | Yarrowia lipolytica |
| 3.1.1.3 | 12260 | - |
purified YLLIP2, substrate olive oil | Yarrowia lipolytica |
| 3.1.1.3 | 16920 | - |
purified YLLIP2, substrate trioctanoin | Yarrowia lipolytica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.3 | olive oil + H2O | - |
Yarrowia lipolytica | ? | - |
? | |
| 3.1.1.3 | soybean oil + H2O | substrate is stabilized with egg yolk lecithin | Yarrowia lipolytica | ? | - |
? | |
| 3.1.1.3 | tributyrin + H2O | - |
Yarrowia lipolytica | ? | - |
? | |
| 3.1.1.3 | trioctanoin + H2O | - |
Yarrowia lipolytica | ? | - |
? | |
| 3.1.1.3 | triolein + H2O | the enzyme shows a preference for the hydrolysis of the sn-3 ester bond of triolein compared to the sn-1 position | Yarrowia lipolytica | 1,2-diolein + oleate | higher amount of 1,2-diolein | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | ? | x * 38000, SDS-PAGE, x * 36874-38481, four glycosylated isozymes, mass spectrometry, x * 33385, nonglycosylated enzyme, mass spectrometry | Yarrowia lipolytica |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | fast lipase | - |
Yarrowia lipolytica |
| 3.1.1.3 | LIP2 | - |
Yarrowia lipolytica |
| 3.1.1.3 | YlLip2 | - |
Yarrowia lipolytica |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 37 | - |
assay at | Yarrowia lipolytica |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 40 | - |
pH 6.0, purified enzyme, half-life is more than 4 h | Yarrowia lipolytica |
| 3.1.1.3 | 50 | - |
pH 6.0, purified enzyme, half-life is 9 min | Yarrowia lipolytica |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 6 | - |
assay at | Yarrowia lipolytica |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 3 | - |
37°C, 1 h, purified enzyme, about 10% remaining activity, complete inactivation after 2 h, half-life 13 min | Yarrowia lipolytica |
| 3.1.1.3 | 4 | 8 | 1 h, 37°C, purified enzyme, 74% remaining activity at pH 4.0, 90% at pH 5.0, 98% at pH 6.0, 88% at pH 7.0, and 48% at pH 8.0, half-life at pH 8.0 is 35 min | Yarrowia lipolytica |
| 3.1.1.3 | 8.5 | - |
37°C, purified enzyme, complete inactivation after 2 h | Yarrowia lipolytica |
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