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Literature summary extracted from

  • Sugimoto, S.; Sugimoto, H.; Aoyama, C.; Aso, C.; Mori, M.; Izumi, T.
    Purification and characterization of lysophospholipase D from rat brain (2006), Biochim. Biophys. Acta, 1761, 1410-1418.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.39 EDTA hydrolysis of 1-palmitoyl-glycerophoshorylcholine is inhibited, indicating that a divalent cation is essential for catalytic activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.39 additional information
-
 additional information suggestion that lysoPLD predominantly utilizes saturated forms of lysophosphatidylcholine Rattus norvegicus
3.1.4.39 0.0083
-
 1-linoleoyl-2-lyso-glycerophoshorylcholine Vmax is 0.019 micromol/min/mg Rattus norvegicus
3.1.4.39 0.0207
-
 1-palmitoyl-2-lyso-glycerophoshorylcholine Vmax is 0.059 micromol/min/mg Rattus norvegicus
3.1.4.39 0.0267
-
 1-O-hexadecyl-2-lyso-glycerophosphorylcholine Vmax is 0.29 micromol/min/mg, lysoPLD activity is more than twice that observed with 1-palmitoyl-glycerophoshorylcholine Rattus norvegicus
3.1.4.39 0.101
-
 1-stearoyl-2-lyso-glycerophoshorylcholine Vmax is 0.035 micromol/min/mg Rattus norvegicus
3.1.4.39 0.176
-
 1-palmitoyl-glycerophoshorylcholine Vmax is 0.3 micromol/min/mg, best substrate Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.39 nucleus
-
 Rattus norvegicus 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.39 Co2+ purified enzyme is incubated with Co2+. LysoPLD activity in serum is more sensitive to Co2+ Rattus norvegicus
3.1.4.39 Mg2+ the purified enzyme is incubated with Mg2+. Purified enzyme is more sensitive to Mg2+ than to Co2+. Enzyme activity requires Mg2+ Rattus norvegicus
3.1.4.39 Mn2+ the purified enzyme is incubated with Mn2+ Rattus norvegicus
3.1.4.39 Na3VO4 enzyme activity requires Na3VO4, not necessary after purification Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.39 35000
-
 lysoPLD, not identical to 100 kDa autotaxin protein Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.39 lysophosphatidylcholine + H2O Rattus norvegicus
-
 lysophosphatidic acid + choline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.39 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.39 includes a protease inhibitor at all steps to avoid isolation of the products of proteolysis. Purified to apparent homogeneity from rat brain nuclear fractions using 1-[14C]palmitoyl-glycerophosphorylcholine as a substrate Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.39 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.39 additional information
-
 nuclear fraction has relatively high levels of lysoPLD activity. Both lysoPLD activity and highest band density found in microsomal fraction Rattus norvegicus
3.1.4.39 0.036
-
 using isotopic assay Rattus norvegicus
3.1.4.39 0.048
-
 colorimetric assay, 6 h incubation, 37°C Rattus norvegicus
3.1.4.39 0.14
-
 purified enzyme, HiTrap DEAE FF Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.39 1-linoleoyl-2-lyso-glycerophoshorylcholine + H2O
-
 Rattus norvegicus 1-linoleoyl-sn-glycerol-3-phosphate + choline
-
 ?
3.1.4.39 1-O-hexadecyl-2-lyso-glycerophosphorylcholine + H2O lysoPAF (alkyl-lysophosphatidylcholine), lysoPLD shows higher activity towards lysoPAF Rattus norvegicus 1-O-hexadecyl-sn-glycerol-3-phosphate + choline
-
 ?
3.1.4.39 1-palmitoyl-2-lyso-glycerophoshorylcholine + H2O
-
 Rattus norvegicus 1-palmitoyl-sn-glycerol-3-phosphate + choline
-
 ?
3.1.4.39 1-stearoyl-2-lyso-glycerophoshorylcholine + H2O
-
 Rattus norvegicus 1-stearoyl-sn-glycerol-3-phosphate + choline
-
 ?
3.1.4.39 lysophosphatidylcholine + H2O
-
 Rattus norvegicus lysophosphatidic acid + choline
-
 ?
3.1.4.39 lysophosphatidylcholine + H2O LPC, purified enzyme hydrolyzes saturated forms of lysophosphatidylcholine more robustly than unsaturated forms Rattus norvegicus lysophosphatic acid + choline
-
 ?
3.1.4.39 platelet-activating factor + H2O PAF, same extent as palmityl-lysophosphatidylcholine Rattus norvegicus ? + choline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.39 monomer
-
 Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.1.4.39 lyso-PLD
-
 Rattus norvegicus
3.1.4.39 lysophospholipase D
-
 Rattus norvegicus
3.1.4.39 lysoPLD purified from rat brain nucleus fractions, has the ability to hydrolyze the ester-bond between phoshate and choline in 1-palmitoyl-glycerophoshorylcholine Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.39 7 7.5 highest enzyme activity Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.39 6 9
-
 Rattus norvegicus