EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.13.2.1 | 15 | - |
S-adenosyl-L-homocysteine | - |
Saccharolobus solfataricus | |
3.13.2.1 | 150 | - |
L-homocysteine | - |
Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.13.2.1 | 47000 | - |
4 * 47000, SDS-PAGE | Saccharolobus solfataricus |
3.13.2.1 | 190000 | - |
gel filtration | Saccharolobus solfataricus |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.13.2.1 | Ethanol | 50%, 48 h at room temperature, no loss of activity | Saccharolobus solfataricus |
3.13.2.1 | guanidine-HCl | 6 M, 48 h at room temperature, no loss of activity | Saccharolobus solfataricus |
3.13.2.1 | Methanol | 50%, 48 h at room temperature, no loss of activity | Saccharolobus solfataricus |
3.13.2.1 | SDS | 0.075%, 48 h at room temperature, no loss of activity | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.13.2.1 | Saccharolobus solfataricus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.13.2.1 | - |
Saccharolobus solfataricus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.13.2.1 | 0.161 | - |
- |
Saccharolobus solfataricus |
EC Number | Storage Stability | Organism |
---|---|---|
3.13.2.1 | -20°C, 20 mM Tris-HC1, pH 8, containing 1 mM EDTA, stable for at least 2 months | Saccharolobus solfataricus |
3.13.2.1 | 4°C, the enzyme is spontaneously inactivated in one month, in the presence of 10% glycerol the stability of the enzyme increases up to 80% | Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.13.2.1 | L-homocysteine + adenosine | - |
Saccharolobus solfataricus | S-adenosyl-L-homocysteine + H2O | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.13.2.1 | tetramer | 4 * 47000, SDS-PAGE | Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.13.2.1 | 75 | - |
- |
Saccharolobus solfataricus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.13.2.1 | 55 | 90 | about 50% of maximal activity 55°C and at 90°C | Saccharolobus solfataricus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.13.2.1 | 25 | - |
60 min, 20% loss of activity | Saccharolobus solfataricus |
3.13.2.1 | 50 | - |
16 h, stable | Saccharolobus solfataricus |
3.13.2.1 | 70 | - |
16 h, 40% loss of activity | Saccharolobus solfataricus |
3.13.2.1 | 75 | - |
60 min, almost complete loss of activity | Saccharolobus solfataricus |
3.13.2.1 | 90 | - |
2 h, 80% loss of activity | Saccharolobus solfataricus |
3.13.2.1 | 95 | - |
melting temperature | Saccharolobus solfataricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.13.2.1 | 7.5 | - |
- |
Saccharolobus solfataricus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
3.13.2.1 | NAD+ | the enzyme contains four molecules of tightly-bound NAD+ per tetramer of which about 40% is in the reduced form | Saccharolobus solfataricus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.13.2.1 | Saccharolobus solfataricus | - |
- |
5.3 |