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Literature summary extracted from

  • Yip, V.L.; Thompson, J.; Withers, S.G.
    Mechanism of GlvA from Bacillus subtilis: a detailed kinetic analysis of a 6-phospho-alpha-glucosidase from glycoside hydrolase family 4 (2007), Biochemistry, 46, 9840-9852.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.122 cellobiose 6'-phosphate competitive inhibitor Bacillus subtilis
3.2.1.122 methyl 6-phospho-alpha-D-glucoside competitive inhibitor Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.122 0.004
-
 3,4-dinitrophenyl 6-phospho-alpha-D-glucoside at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis
3.2.1.122 0.012
-
 3,4-dinitrophenyl 6-phospho-alpha-D-glucoside at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis
3.2.1.122 0.014
-
 4-nitrophenyl 6-phospho-alpha-D-glucoside at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis
3.2.1.122 0.038
-
 phenyl 6-phospho-alpha-D-glucoside at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis
3.2.1.122 0.052
-
 4-nitrophenyl 6-phospho-alpha-D-glucoside at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis
3.2.1.122 0.069
-
 phenyl 6-phospho-alpha-D-glucoside at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis
3.2.1.122 0.36
-
 maltose 6'-phosphate at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis
3.2.1.122 0.4
-
 maltose 6'-phosphate at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis
3.2.1.122 0.5
-
 methyl 6-phospho-alpha-D-glucoside at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis
3.2.1.122 0.61
-
 methyl 6-phospho-alpha-D-glucoside at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.122 Mn2+
-
 Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.122 Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.122 3,4-dinitrophenyl 6-phospho-alpha-D-glucoside + H2O
-
 Bacillus subtilis 3,4-dinitrophenol + alpha-D-glucose 6-phosphate
-
 ?
3.2.1.122 4-nitrophenyl 6-phospho-alpha-D-glucoside + H2O
-
 Bacillus subtilis 4-nitrophenol + alpha-D-glucose
-
 ?
3.2.1.122 4-nitrophenyl 6-phospho-beta-D-glucoside + H2O
-
 Bacillus subtilis 4-nitrophenol + beta-D-glucose 6-phosphate
-
 ?
3.2.1.122 maltose 6'-phosphate + H2O
-
 Bacillus subtilis D-glucose 6-phosphate + D-glucose
-
 ?
3.2.1.122 methyl 6-phospho-alpha-D-glucoside + H2O
-
 Bacillus subtilis methanol + alpha-D-glucose 6-phosphate
-
 ?
3.2.1.122 phenyl 6-phospho-alpha-D-glucoside + H2O
-
 Bacillus subtilis phenol + alpha-D-glucose 6-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.122 6-phospho-alpha-glucosidase
-
 Bacillus subtilis
3.2.1.122 GlvA
-
 Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.122 0.26
-
 phenyl 6-phospho-alpha-D-glucoside at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis
3.2.1.122 0.42
-
 methyl 6-phospho-alpha-D-glucoside at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis
3.2.1.122 0.7
-
 4-nitrophenyl 6-phospho-alpha-D-glucoside at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis
3.2.1.122 0.72
-
 3,4-dinitrophenyl 6-phospho-alpha-D-glucoside at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis
3.2.1.122 0.81
-
 3,4-dinitrophenyl 6-phospho-alpha-D-glucoside at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis
3.2.1.122 0.85
-
 4-nitrophenyl 6-phospho-alpha-D-glucoside at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis
3.2.1.122 0.9
-
 phenyl 6-phospho-alpha-D-glucoside at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis
3.2.1.122 1.1
-
 methyl 6-phospho-alpha-D-glucoside at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis
3.2.1.122 1.3
-
 maltose 6'-phosphate at 37°C in HEPES buffer, pH 7.5 Bacillus subtilis
3.2.1.122 1.3
-
 maltose 6'-phosphate at 37°C in Tris-HCl buffer, pH 8.4 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.122 4 10
-
 Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
3.2.1.122 additional information not activated by NADH Bacillus subtilis
3.2.1.122 NAD+
-
 Bacillus subtilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.122 0.03
-
 cellobiose 6'-phosphate
-
 Bacillus subtilis
3.2.1.122 0.075
-
 methyl 6-phospho-alpha-D-glucoside
-
 Bacillus subtilis