EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.22.70 | wild-type and variant SrtA proteins | Staphylococcus aureus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.22.70 | C184S | 2700fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
3.4.22.70 | D185A 1.3 | fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
3.4.22.70 | D186A | 1.8fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
3.4.22.70 | H120A | 96000fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
3.4.22.70 | H120Q | 36000fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
3.4.22.70 | I182A | mutation produces modest decreases in SrtA activity and leds to substrate inhibition. 28fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
3.4.22.70 | I182S | 74fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
3.4.22.70 | L181A | mutation produces modest decreases in SrtA activity and leds to substrate inhibition. 7.6fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
3.4.22.70 | R197A | 540fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
3.4.22.70 | R197K | 1000fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
3.4.22.70 | T180A | mutation produces modest decreases in SrtA activity and leds to substrate inhibition. 14fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
3.4.22.70 | T183A | 1200fold decrease in kcat/Km compared to wild-type value | Staphylococcus aureus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.70 | 0.79 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
3.4.22.70 | 3.8 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
3.4.22.70 | 4.7 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
3.4.22.70 | 6.7 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
3.4.22.70 | 8.5 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
3.4.22.70 | 10.6 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | wild-type enzyme | Staphylococcus aureus | |
3.4.22.70 | 11.2 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.70 | Staphylococcus aureus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.70 | - |
Staphylococcus aureus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.70 | Abz-LPETG-Dap(Dnp)-NH2 + GGGGG | - |
Staphylococcus aureus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.22.70 | SrtA | - |
Staphylococcus aureus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.70 | 0.000019 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
3.4.22.70 | 0.0003 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
3.4.22.70 | 0.00046 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
3.4.22.70 | 0.0006 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
3.4.22.70 | 0.69 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | wild-type enzyme | Staphylococcus aureus | |
3.4.22.70 | 0.72 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus | |
3.4.22.70 | 0.78 | - |
N-(2-aminobenzoyl)-L-leucyl-L-prolyl-L-alpha-glutamyl-L-threonylglycyl-3-(2,4-dinitroanilino)-L-alaninamide | mutant enzyme | Staphylococcus aureus |