EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.22 | mutant enzymes K102N, P78R and P78R/K102N are expressed in Escherichia coli and purified as glutathione S-transferase fusion proteins using recombinant expression | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.22 | K102N | KM for L-serine is about 2fold higher than wild-type value. KM for L-homocysteine is 2fold higher than wild-type value | Homo sapiens |
4.2.1.22 | P78R | KM for L-serine is about 4fold higher than wild-type value. KM for L-homocysteine is comparable to wild-type value | Homo sapiens |
4.2.1.22 | P78R/K102N | KM for L-serine is about 2fold higher than wild-type value. Mutant enzyme is insensitive to allosteric regulation and unresponsive to S-adenosyl-L-methionine | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.22 | Homo sapiens | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.22 | L-serine + L-homocysteine | - |
Homo sapiens | cystathionine + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.22 | More | mutant enzymes K102N and P78R/K102N behaves like wild-type enzyme by native gel chromatography and exist as a mixture of higher-order quaternary states | Homo sapiens |