Literature summary extracted from

Shishova, E.Y.; Di Costanzo, L.; Cane, D.E.; Christianson, D.W.
X-ray crystal structure of aristolochene synthase from Aspergillus terreus and evolution of templates for the cyclization of farnesyl diphosphate (2007), Biochemistry, 46, 1941-1951.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.2.3.9	into the pET11 vector for expression in Escherichia coli BL21DE3pLysS cells	Aspergillus terreus

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
4.2.3.9	the structure of recombinant aristolochene synthase at a resolution of 2.2 A and its complex with diphosphate and three Mg2+ ions at 2.15 A is reported	Aspergillus terreus

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
4.2.3.9	Mg2+	-	Aspergillus terreus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
4.2.3.9	70000	-	dimer, native gel analysis	Aspergillus terreus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4.2.3.9	2-trans,6-trans-farnesyl diphosphate	Aspergillus terreus	-	aristolochene + diphosphate	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.2.3.9	Aspergillus terreus	Q9UR08	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
4.2.3.9	using DE52 anion exchange resin, a methyl HIC, a Sephadex G-25, and a High Q column	Aspergillus terreus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.2.3.9	2-trans,6-trans-farnesyl diphosphate	-	Aspergillus terreus	aristolochene + diphosphate	-	?

Subunits

EC Number	Subunits	Comment	Organism
4.2.3.9	dimer	-	Aspergillus terreus

Synonyms

EC Number	Synonyms	Comment	Organism
4.2.3.9	aristolochene synthase	-	Aspergillus terreus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)