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Literature summary extracted from

  • Mukherjee, T.; McCulloch, K.M.; Ealick, S.E.; Begley, T.P.
    Gene identification and structural characterization of the pyridoxal 5-phosphate degradative protein 3-hydroxy-2-methylpyridine-4,5-dicarboxylate decarboxylase from Mesorhizobium loti MAFF303099 (2007), Biochemistry, 46, 13606-13615.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.51 expressed in Escherichia coli strain BL21(DE3) Mesorhizobium loti

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.51 hanging drop vapour diffusion method, optimized crystallization conditions consist of 6-9% polyethylene glycol 8000 and 100 mM Tris buffer with a pH ranging from 7.0 to 7.5 Mesorhizobium loti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.51 0.366
-
 3-hydroxy-2-methylpyridine-4,5-dicarboxylate in 100 mM Tris-HCl at pH 8.0 containing 100 mM NaCl and 2 mM dithiothreitol Mesorhizobium loti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.51 26000
-
 SDS-PAGE Mesorhizobium loti

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.51 Mesorhizobium loti
-
-
-
4.1.1.51 Mesorhizobium loti MAFF303099
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.51 Ni-NTA affinity chromatography Mesorhizobium loti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.51 3-Hydroxy-2-methylpyridine-4,5-dicarboxylate
-
 Mesorhizobium loti 3-Hydroxy-2-methylpyridine-5-carboxylate + CO2
-
 ?
4.1.1.51 3-Hydroxy-2-methylpyridine-4,5-dicarboxylate
-
 Mesorhizobium loti MAFF303099 3-Hydroxy-2-methylpyridine-5-carboxylate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.51 tetramer x-ray crystallography Mesorhizobium loti

Synonyms

EC Number Synonyms Comment Organism
4.1.1.51 3-hydroxy-2-methylpyridine-4,5-dicarboxylate decarboxylase
-
 Mesorhizobium loti
4.1.1.51 HMPDdc the enzyme is involved in the catabolism of pyridoxal 5'-phosphate Mesorhizobium loti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.51 0.6
-
 3-hydroxy-2-methylpyridine-4,5-dicarboxylate in 100 mM Tris-HCl at pH 8.0 containing 100 mM NaCl and 2 mM dithiothreitol Mesorhizobium loti