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Literature summary extracted from

  • Yip, V.L.; Withers, S.G.
    Mechanistic analysis of the unusual redox-elimination sequence employed by Thermotoga maritima BglT: a 6-phospho-beta-glucosidase from glycoside hydrolase family 4 (2006), Biochemistry, 45, 571-580.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.86 0.015
-
4-chloro-2-nitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 0.0166
-
2,5-dinitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 0.0174
-
2-nitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 0.0314
-
3,4-dinitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 0.0319
-
4-cyanophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 0.0414
-
3,5-dichlorophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 0.0444
-
2,4-dinitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 0.0454
-
4-cyanophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 0.048
-
4-nitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 0.0583
-
4-tert-butylphenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 0.0729
-
3-nitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.86 Thermotoga maritima
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.86 2,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
-
Thermotoga maritima 2,4-dinitrophenol + D-glucose 6-phosphate
-
?
3.2.1.86 2,5-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
-
Thermotoga maritima 2,5-dinitrophenol + D-glucose 6-phosphate
-
?
3.2.1.86 2-nitrophenyl 6-phospho-beta-D-glucoside + H2O
-
Thermotoga maritima 2-nitrophenol + D-glucose 6-phosphate
-
?
3.2.1.86 3,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
-
Thermotoga maritima 3,4-dinitrophenol + D-glucose 6-phosphate
-
?
3.2.1.86 3,5-dichlorophenyl 6-phospho-beta-D-glucoside + H2O
-
Thermotoga maritima 3,5-dichlorophenol + D-glucose 6-phosphate
-
?
3.2.1.86 3-nitrophenyl 6-phospho-beta-D-glucoside + H2O
-
Thermotoga maritima 3-nitrophenol + D-glucose 6-phosphate
-
?
3.2.1.86 4-chloro-2-nitrophenyl 6-phospho-beta-D-glucoside + H2O
-
Thermotoga maritima 4-chloro-2-nitrophenol + D-glucose 6-phosphate
-
?
3.2.1.86 4-cyanophenyl 6-phospho-beta-D-glucoside + H2O
-
Thermotoga maritima 4-cyanophenol + D-glucose 6-phosphate
-
?
3.2.1.86 4-nitrophenyl 6-phospho-beta-D-glucoside + H2O
-
Thermotoga maritima 4-nitrophenol + D-glucose 6-phosphate
-
?
3.2.1.86 4-tert-butylphenyl 6-phospho-beta-D-glucoside + H2O
-
Thermotoga maritima 4-tert-butylphenol + D-glucose 6-phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.86 6-phospho-beta-glucosidase
-
Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.86 0.79
-
4-cyanophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 0.99
-
4-nitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 1.01
-
4-cyanophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 1.15
-
3,4-dinitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 1.21
-
2-nitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 1.21
-
4-tert-butylphenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 1.69
-
4-chloro-2-nitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 1.95
-
2,5-dinitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 2.29
-
2,4-dinitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 2.33
-
3,5-dichlorophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 4.69
-
3-nitrophenyl 6-phospho-beta-D-glucoside pH 7.5, 50°C Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
3.2.1.86 NAD+ enzyme is completely inactive in the absence of NAD+. Kd-value is 480 nM Thermotoga maritima