EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.3.1.1 | mutant P168A, with and without 2-phosphoglycolate. Phosphate moiety of 2-phosphoglycolate is bound similarly as in wild-type, but interactions of the carboxylic acid moiety with the side chain of catalytic Glu167 differ | Trypanosoma brucei |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.3.1.1 | P168A | mutation beside catalytic residue Glu167. Mutant turnover number is 50fold reduced, Km value is 2fold reduced | Trypanosoma brucei |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.1 | 2-Phosphoglycolate | - |
Trypanosoma brucei | |
5.3.1.1 | AsO43- | - |
Trypanosoma brucei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.1 | 0.16 | - |
D-glyceraldehyde 3-phosphate | mutant P168A, 25°C, pH 7.6 | Trypanosoma brucei | |
5.3.1.1 | 0.26 | - |
D-glyceraldehyde 3-phosphate | wild-type, 25°C, pH 7.6 | Trypanosoma brucei | |
5.3.1.1 | 0.5 | - |
dihydroxyacetone phosphate | mutant P168A, 25°C, pH 7.6 | Trypanosoma brucei | |
5.3.1.1 | 0.9 | - |
dihydroxyacetone phosphate | wild-type, 25°C, pH 7.6 | Trypanosoma brucei |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.1 | Trypanosoma brucei | P04789 | - |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
5.3.1.1 | D-Glyceraldehyde 3-phosphate = glycerone phosphate | upon binding of ligand, the side chain of Glu167 flips from the inactive swung-out to the active swung-in conformation. A concerted movement of loop 6 and loop 7 from unliganded-open to liganded-closed is facilitated by the interactions of the phosphate moiety with loop 7. Rotation of the Gly211-Gly212 peptide plane of 90° is involved in this concerted movement | Trypanosoma brucei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.1 | D-glyceraldehyde 3-phosphate | - |
Trypanosoma brucei | dihydroxyacetone phosphate | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.1 | 19 | - |
dihydroxyacetone phosphate | mutant P168A, 25°C, pH 7.6 | Trypanosoma brucei | |
5.3.1.1 | 60 | - |
D-glyceraldehyde 3-phosphate | mutant P168A, 25°C, pH 7.6 | Trypanosoma brucei | |
5.3.1.1 | 645 | - |
dihydroxyacetone phosphate | wild-type, 25°C, pH 7.6 | Trypanosoma brucei | |
5.3.1.1 | 3570 | - |
D-glyceraldehyde 3-phosphate | wild-type, 25°C, pH 7.6 | Trypanosoma brucei |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.1 | 0.05 | - |
2-Phosphoglycolate | wild-type, 25°C, pH 7.6 | Trypanosoma brucei | |
5.3.1.1 | 0.3 | - |
2-Phosphoglycolate | mutant P168A, 25°C, pH 7.6 | Trypanosoma brucei | |
5.3.1.1 | 4.9 | - |
AsO43- | wild-type, 25°C, pH 7.6 | Trypanosoma brucei | |
5.3.1.1 | 5.8 | - |
AsO43- | mutant P168A, 25°C, pH 7.6 | Trypanosoma brucei |