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Literature summary extracted from

  • Wang, Y.; Li, Y.; Yan, H.
    Mechanism of dihydroneopterin aldolase: functional roles of the conserved active site glutamate and lysine residues (2006), Biochemistry, 45, 15232-15239.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.25 E21A strongly reduced kcat Escherichia coli
4.1.2.25 E22A strongly reduced kcat Staphylococcus aureus
4.1.2.25 E73A strongly reduced kcat Escherichia coli
4.1.2.25 E74A mutation causes dramatic changes in the affinities of the enzyme for the substrate or product analogues or the rate constants Staphylococcus aureus
4.1.2.25 K100A strongly reduced kcat Staphylococcus aureus
4.1.2.25 K100Q strongly reduced kcat Staphylococcus aureus
4.1.2.25 K98A strongly reduced kcat Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.25 0.00076
-
 7,8-dihydro-L-monapterin mutant enzyme E21A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
4.1.2.25 0.0016
-
 7,8-dihydroneopterin mutant enzyme E21A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
4.1.2.25 0.0024
-
 7,8-dihydroneopterin mutant enzyme K98A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
4.1.2.25 0.0029
-
 7,8-dihydro-L-monapterin mutant enzyme K98A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
4.1.2.25 0.0039
-
 7,8-dihydroneopterin mutant enzyme E22A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
4.1.2.25 0.004
-
 7,8-dihydro-L-monapterin mutant enzyme E22A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
4.1.2.25 0.0046
-
 7,8-dihydroneopterin wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
4.1.2.25 0.0055
-
 7,8-dihydro-L-monapterin wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
4.1.2.25 0.0058
-
 7,8-dihydroneopterin mutant enzyme K100A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
4.1.2.25 0.0074
-
 7,8-dihydroneopterin wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
4.1.2.25 0.0095
-
 7,8-dihydro-L-monapterin mutant enzyme K100A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
4.1.2.25 8
-
 7,8-dihydro-L-monapterin wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
4.1.2.25 9.7
-
 7,8-dihydroneopterin mutant enzyme E73A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
4.1.2.25 9.8
-
 7,8-dihydro-L-monapterin mutant enzyme E73A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.25 Escherichia coli
-
-
-
4.1.2.25 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.25 DEAE-cellulose column chromatography and Bio-Gel A-0.5m gel filtration Escherichia coli
4.1.2.25 Ni-NTA column chromatography and Bio-Gel A-0.5m gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.25 6-hydroxymethyl-7,8-dihydropterin
-
 Staphylococcus aureus ?
-
 ?
4.1.2.25 6-hydroxymethyl-7,8-dihydropterin
-
 Escherichia coli ?
-
 ?
4.1.2.25 7,8-dihydro-L-monapterin
-
 Staphylococcus aureus ?
-
 ?
4.1.2.25 7,8-dihydro-L-monapterin
-
 Escherichia coli ?
-
 ?
4.1.2.25 7,8-dihydroneopterin
-
 Staphylococcus aureus 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde
-
 ?
4.1.2.25 7,8-dihydroneopterin
-
 Escherichia coli 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.25 DHNA
-
 Staphylococcus aureus
4.1.2.25 DHNA
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.25 0.0000022
-
 7,8-dihydroneopterin mutant enzyme K100A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
4.1.2.25 0.0000023
-
 7,8-dihydro-L-monapterin mutant enzyme E21A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
4.1.2.25 0.0000043
-
 7,8-dihydroneopterin mutant enzyme K98A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
4.1.2.25 0.0000051
-
 7,8-dihydro-L-monapterin mutant enzyme K100A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
4.1.2.25 0.0000053
-
 7,8-dihydro-L-monapterin mutant enzyme K98A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
4.1.2.25 0.0000057
-
 7,8-dihydro-L-monapterin mutant enzyme K100Q, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
4.1.2.25 0.000006
-
 7,8-dihydro-L-monapterin mutant enzyme E73A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
4.1.2.25 0.0000065
-
 7,8-dihydroneopterin mutant enzyme E21A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
4.1.2.25 0.0000065
-
 7,8-dihydro-L-monapterin mutant enzyme E22A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
4.1.2.25 0.0000093
-
 7,8-dihydroneopterin mutant enzyme E22A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
4.1.2.25 0.000016
-
 7,8-dihydroneopterin mutant enzyme K100Q, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
4.1.2.25 0.00073
-
 7,8-dihydroneopterin mutant enzyme E73A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
4.1.2.25 0.01
-
 7,8-dihydro-L-monapterin wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
4.1.2.25 0.045
-
 7,8-dihydroneopterin wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Staphylococcus aureus
4.1.2.25 0.082
-
 7,8-dihydroneopterin wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli
4.1.2.25 0.089
-
 7,8-dihydro-L-monapterin wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.25 9.6
-
-
 Staphylococcus aureus
4.1.2.25 9.6
-
-
 Escherichia coli