BRENDA - Enzyme Database

Evolution of enzymatic activities in the enolase superfamily: L-fuconate dehydratase from Xanthomonas campestris

Yew, W.S.; Fedorov, A.A.; Fedorov, E.V.; Rakus, J.F.; Pierce, R.W.; Almo, S.C.; Gerlt, J.A.; Biochemistry 45, 14582-14597 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.68
-
Xanthomonas campestris
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.68
0.33
-
L-fuconate
25°C, pH 7.5
Xanthomonas campestris
4.2.1.68
2.1
-
D-Arabinonate
25°C, pH 7.5
Xanthomonas campestris
4.2.1.68
6.2
-
L-galactonate
25°C, pH 7.5
Xanthomonas campestris
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.68
Xanthomonas campestris
-
pv. campestris str. ATCC 33913
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.68
D-altronate
-
678170
Xanthomonas campestris
?
-
-
-
?
4.2.1.68
D-arabinonate
dehydration of D-arabinonate is initiated by abstraction of the 2-proton by Lys220
678170
Xanthomonas campestris
?
-
-
-
?
4.2.1.68
D-ribonate
dehydrations of D-ribonate is initiated by abstraction of the 2-proton by His351
678170
Xanthomonas campestris
?
-
-
-
?
4.2.1.68
L-fuconate
FucD reaction involves initial abstraction of the 2-proton by Lys220, acid catalysis of the vinylogous beta-elimination of the 3-OH group by His351, and stereospecific ketonization of the resulting enol, likely by the conjugate acid of Lys220, to yield the 2-keto-3-deoxy-L-fuconate product
678170
Xanthomonas campestris
2-dehydro-3-deoxy-L-fuconate + H2O
-
-
-
?
4.2.1.68
L-galactonate
dehydration of L-galactonate is initiated by abstraction of the 2-proton by Lys220
678170
Xanthomonas campestris
2-keto-3-deoxy-L-galactonate + H2O
-
-
-
?
4.2.1.68
L-talonate
dehydrations of L-talonate is initiated by abstraction of the 2-proton by His351
678170
Xanthomonas campestris
?
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.68
0.9
-
L-galactonate
25°C, pH 7.5
Xanthomonas campestris
4.2.1.68
2.4
-
D-Arabinonate
25°C, pH 7.5
Xanthomonas campestris
4.2.1.68
15
-
L-fuconate
25°C, pH 7.5
Xanthomonas campestris
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.68
-
Xanthomonas campestris
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.68
0.33
-
L-fuconate
25°C, pH 7.5
Xanthomonas campestris
4.2.1.68
2.1
-
D-Arabinonate
25°C, pH 7.5
Xanthomonas campestris
4.2.1.68
6.2
-
L-galactonate
25°C, pH 7.5
Xanthomonas campestris
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.68
D-altronate
-
678170
Xanthomonas campestris
?
-
-
-
?
4.2.1.68
D-arabinonate
dehydration of D-arabinonate is initiated by abstraction of the 2-proton by Lys220
678170
Xanthomonas campestris
?
-
-
-
?
4.2.1.68
D-ribonate
dehydrations of D-ribonate is initiated by abstraction of the 2-proton by His351
678170
Xanthomonas campestris
?
-
-
-
?
4.2.1.68
L-fuconate
FucD reaction involves initial abstraction of the 2-proton by Lys220, acid catalysis of the vinylogous beta-elimination of the 3-OH group by His351, and stereospecific ketonization of the resulting enol, likely by the conjugate acid of Lys220, to yield the 2-keto-3-deoxy-L-fuconate product
678170
Xanthomonas campestris
2-dehydro-3-deoxy-L-fuconate + H2O
-
-
-
?
4.2.1.68
L-galactonate
dehydration of L-galactonate is initiated by abstraction of the 2-proton by Lys220
678170
Xanthomonas campestris
2-keto-3-deoxy-L-galactonate + H2O
-
-
-
?
4.2.1.68
L-talonate
dehydrations of L-talonate is initiated by abstraction of the 2-proton by His351
678170
Xanthomonas campestris
?
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.68
0.9
-
L-galactonate
25°C, pH 7.5
Xanthomonas campestris
4.2.1.68
2.4
-
D-Arabinonate
25°C, pH 7.5
Xanthomonas campestris
4.2.1.68
15
-
L-fuconate
25°C, pH 7.5
Xanthomonas campestris