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Literature summary extracted from

  • Yew, W.S.; Fedorov, A.A.; Fedorov, E.V.; Rakus, J.F.; Pierce, R.W.; Almo, S.C.; Gerlt, J.A.
    Evolution of enzymatic activities in the enolase superfamily: L-fuconate dehydratase from Xanthomonas campestris (2006), Biochemistry, 45, 14582-14597.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.68
-
 Xanthomonas campestris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.68 0.33
-
 L-fuconate 25°C, pH 7.5 Xanthomonas campestris
4.2.1.68 2.1
-
 D-Arabinonate 25°C, pH 7.5 Xanthomonas campestris
4.2.1.68 6.2
-
 L-galactonate 25°C, pH 7.5 Xanthomonas campestris

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.68 Xanthomonas campestris
-
 pv. campestris str. ATCC 33913
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.68 D-altronate
-
 Xanthomonas campestris ?
-
 ?
4.2.1.68 D-arabinonate dehydration of D-arabinonate is initiated by abstraction of the 2-proton by Lys220 Xanthomonas campestris ?
-
 ?
4.2.1.68 D-ribonate dehydrations of D-ribonate is initiated by abstraction of the 2-proton by His351 Xanthomonas campestris ?
-
 ?
4.2.1.68 L-fuconate FucD reaction involves initial abstraction of the 2-proton by Lys220, acid catalysis of the vinylogous beta-elimination of the 3-OH group by His351, and stereospecific ketonization of the resulting enol, likely by the conjugate acid of Lys220, to yield the 2-keto-3-deoxy-L-fuconate product Xanthomonas campestris 2-dehydro-3-deoxy-L-fuconate + H2O
-
 ?
4.2.1.68 L-galactonate dehydration of L-galactonate is initiated by abstraction of the 2-proton by Lys220 Xanthomonas campestris 2-keto-3-deoxy-L-galactonate + H2O
-
 ?
4.2.1.68 L-talonate dehydrations of L-talonate is initiated by abstraction of the 2-proton by His351 Xanthomonas campestris ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.68 FucD
-
 Xanthomonas campestris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.68 0.9
-
 L-galactonate 25°C, pH 7.5 Xanthomonas campestris
4.2.1.68 2.4
-
 D-Arabinonate 25°C, pH 7.5 Xanthomonas campestris
4.2.1.68 15
-
 L-fuconate 25°C, pH 7.5 Xanthomonas campestris