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Literature summary extracted from

  • Feng, H.; Dong, L.; Cao, W.
    Catalytic Mechanism of Endonuclease V: A Catalytic and Regulatory Two-Metal Model (2006), Biochemistry, 45, 10251-10259.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.21.7 D110A little cleavage activity Thermotoga maritima
3.1.21.7 D110C mutant Thermotoga maritima
3.1.21.7 D110E mutant Thermotoga maritima
3.1.21.7 D110H mutant Thermotoga maritima
3.1.21.7 D43A no more than 10% cleavage activity Thermotoga maritima
3.1.21.7 D43C no more than 10% cleavage activity Thermotoga maritima
3.1.21.7 D43E no more than 10% cleavage activity Thermotoga maritima
3.1.21.7 D43H no more than 10% cleavage activity Thermotoga maritima
3.1.21.7 E89A about 20-30% cleavage activity Thermotoga maritima
3.1.21.7 E89D mutant Thermotoga maritima
3.1.21.7 E89H mutant Thermotoga maritima
3.1.21.7 H214A tolerant of mutation without impacting activity Thermotoga maritima
3.1.21.7 H214C tolerant of mutation without impacting activity Thermotoga maritima
3.1.21.7 H214D little change in substrate specificity or DNA cleavage kinetics Thermotoga maritima
3.1.21.7 H214D tolerant of mutation without impacting activity Thermotoga maritima
3.1.21.7 H214E tolerant of mutation without impacting activity Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.7 Ca2+ no catalytic function, supports binding to DNA Thermotoga maritima
3.1.21.7 Mg2+
-
 Thermotoga maritima
3.1.21.7 Mn2+ in the presence of Mn2+, 3'-exonuclease activity is pronounced in wild type and mutant enzymes E89D, D110C, H214C, H214E, and H214D, while reduced activity is detected in D110A, D110H, and D110E Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.7 Thermotoga maritima Q9X2H9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.7 DNA + H2O hydrolysis of the second phosphodiester bond 3' from a deaminated base lesion including inosine, xanthosine, oxanosine and uridine Thermotoga maritima hydrolysed DNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.7 endonuclease V
-
 Thermotoga maritima