EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.99.31 | R277G | reduced activity compared to the wild type enzyme, the mutation at Arg277 has no influence on the binding affinity of ester substrates | Pseudomonas putida |
1.1.99.31 | R277K | reduced activity compared to the wild type enzyme, the mutation at Arg277 has no influence on the binding affinity of ester substrates | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.31 | (S)-1-phenyl-1,2-ethanediol | weak inhibitor | Pseudomonas putida | |
1.1.99.31 | (S)-1-phenyl-2,2,2-trifluoroethanol | competitive inhibitor | Pseudomonas putida | |
1.1.99.31 | (S)-1-phenyl-2-propen-1-ol | - |
Pseudomonas putida | |
1.1.99.31 | (S)-1-phenyl-2-propyn-1-ol | - |
Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.31 | 0.12 | - |
(S)-Mandelate | wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida | |
1.1.99.31 | 2.7 | - |
ethyl-(S)-mandelate | mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida | |
1.1.99.31 | 2.9 | - |
ethyl-(S)-mandelate | wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida | |
1.1.99.31 | 3.7 | - |
ethyl-(S)-mandelate | mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida | |
1.1.99.31 | 5.6 | - |
(S)-Mandelate | mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida | |
1.1.99.31 | 7.4 | - |
methyl-(S)-mandelate | mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida | |
1.1.99.31 | 10.3 | - |
methyl-(S)-mandelate | mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida | |
1.1.99.31 | 11.3 | - |
methyl-(S)-mandelate | wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida | |
1.1.99.31 | 17 | - |
(S)-Mandelate | mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.31 | Pseudomonas putida | P20932 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.31 | - |
Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.31 | (S)-mandelate + 2 ferricyanide | - |
Pseudomonas putida | 2-oxo-2-phenylacetate + 2 ferrocyanide + 2 H+ | - |
? | |
1.1.99.31 | ethyl-(S)-mandelate + acceptor | - |
Pseudomonas putida | ? | - |
? | |
1.1.99.31 | methyl-(S)-mandelate + acceptor | - |
Pseudomonas putida | ? | - |
? | |
1.1.99.31 | additional information | (S)-mandelamide, (S)-1-phenyl-2-propen-1-ol, (S)-1-phenyl-1,2-ethanediol, (S)-1-phenyl-2-propyn-1-ol, (R,S)-pantoyllactone, and (S)-1-phenyl-2,2,2-trifluoroethanol are no substrates | Pseudomonas putida | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.99.31 | MDH | - |
Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.31 | 0.23 | - |
(S)-Mandelate | mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida | |
1.1.99.31 | 0.3 | - |
ethyl-(S)-mandelate | mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida | |
1.1.99.31 | 2.3 | - |
methyl-(S)-mandelate | mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida | |
1.1.99.31 | 2.5 | - |
methyl-(S)-mandelate | mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida | |
1.1.99.31 | 2.6 | - |
ethyl-(S)-mandelate | mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida | |
1.1.99.31 | 66 | - |
(S)-Mandelate | mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida | |
1.1.99.31 | 151 | - |
methyl-(S)-mandelate | wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida | |
1.1.99.31 | 204 | - |
ethyl-(S)-mandelate | wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida | |
1.1.99.31 | 360 | - |
(S)-Mandelate | wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.31 | FMN | dependent | Pseudomonas putida |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.31 | 2.6 | - |
(S)-1-phenyl-2,2,2-trifluoroethanol | wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida | |
1.1.99.31 | 53.8 | - |
(S)-1-phenyl-1,2-ethanediol | wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 | Pseudomonas putida |