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Literature summary extracted from

  • Giordano, A.; Cannio, R.; La Cara, F.; Bartolucci, S.; Rossi, M.; Raia, C.A.
    Asn249Tyr substitution at the coenzyme binding domain activates Sulfolobus solfataricus alcohol dehydrogenase and increases its thermal stability. (1999), Biochemistry, 38, 3043-3054.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 expressed in Escherichia coli RB791 cells Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.1 N249Y mutant shows increased thermal stability and increased catalytic activity compared to the wild type enzyme Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 guanidine hydrochloride
-
 Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.013
-
 NADH wild type enzyme, at 65°C, in the presence of benzaldehyde Saccharolobus solfataricus
1.1.1.1 0.024
-
 4-methoxybenzaldehyde wild type enzyme, at 65°C Saccharolobus solfataricus
1.1.1.1 0.03
-
 benzaldehyde wild type enzyme, at 65°C Saccharolobus solfataricus
1.1.1.1 0.03
-
 Cyclohexanol wild type enzyme, at 65°C Saccharolobus solfataricus
1.1.1.1 0.21
-
 NADH mutant enzyme N249Y, at 65°C, in the presence of benzaldehyde Saccharolobus solfataricus
1.1.1.1 0.23
-
 4-methoxybenzyl alcohol wild type enzyme, at 65°C Saccharolobus solfataricus
1.1.1.1 0.26
-
 benzaldehyde mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
1.1.1.1 0.26
-
 benzyl alcohol wild type enzyme, at 65°C Saccharolobus solfataricus
1.1.1.1 0.5
-
 NAD+ wild type enzyme, at 65°C, in the presence of benzyl alcohol Saccharolobus solfataricus
1.1.1.1 0.6
-
 1-propanol wild type enzyme, at 65°C Saccharolobus solfataricus
1.1.1.1 0.86
-
 4-methoxybenzaldehyde mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
1.1.1.1 1.24
-
 4-methoxybenzyl alcohol mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
1.1.1.1 1.5
-
 benzyl alcohol mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
1.1.1.1 1.7
-
 1-propanol mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
1.1.1.1 1.9
-
 Cyclohexanol mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
1.1.1.1 12.4
-
 NAD+ mutant enzyme N249Y, at 65°C, in the presence of benzyl alcohol Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 37585
-
 4 * 37585, electrospray mass spectrometry Saccharolobus solfataricus
1.1.1.1 150300
-
 electrospray mass spectrometry Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1 DEAE-Sepharose Fast Flow column chromatography and G75 gel filtration Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 4.1
-
 wild type enzyme, at 65°C Saccharolobus solfataricus
1.1.1.1 26.1
-
 mutant enzyme N249Y, at 65°C Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 1-propanol + NAD+
-
 Saccharolobus solfataricus propanal + NADH + H+
-
 ?
1.1.1.1 4-methoxybenzyl alcohol + NAD+
-
 Saccharolobus solfataricus 4-methoxybenzaldehyde + NADH + H+
-
 r
1.1.1.1 benzyl alcohol + NAD+
-
 Saccharolobus solfataricus benzaldehyde + NADH + H+
-
 r
1.1.1.1 cyclohexanol + NAD+
-
 Saccharolobus solfataricus cyclohexanone + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 homotetramer 4 * 37585, electrospray mass spectrometry Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 ADH
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 70 80 wild type and mutant enzyme N249Y are stable at 70°C, the mutant enzyme seems more thermoresistant than the wild type enzyme up to a temperature of 80°C, after which its activity decreases abruptly Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.1 1.2
-
 Cyclohexanol wild type enzyme, at 65°C Saccharolobus solfataricus
1.1.1.1 2.6
-
 1-propanol wild type enzyme, at 65°C Saccharolobus solfataricus
1.1.1.1 3.3
-
 4-methoxybenzaldehyde wild type enzyme, at 65°C Saccharolobus solfataricus
1.1.1.1 3.3
-
 4-methoxybenzyl alcohol wild type enzyme, at 65°C Saccharolobus solfataricus
1.1.1.1 3.4
-
 NAD+ wild type enzyme, at 65°C, in the presence of benzyl alcohol Saccharolobus solfataricus
1.1.1.1 3.6
-
 benzyl alcohol wild type enzyme, at 65°C Saccharolobus solfataricus
1.1.1.1 10.3
-
 NADH wild type enzyme, at 65°C, in the presence of benzaldehyde Saccharolobus solfataricus
1.1.1.1 11.3
-
 benzaldehyde wild type enzyme, at 65°C Saccharolobus solfataricus
1.1.1.1 15.9
-
 Cyclohexanol mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
1.1.1.1 16.6
-
 1-propanol mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
1.1.1.1 20.5
-
 4-methoxybenzaldehyde mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
1.1.1.1 21.7
-
 benzyl alcohol mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
1.1.1.1 25.3
-
 NADH mutant enzyme N249Y, at 65°C, in the presence of benzaldehyde Saccharolobus solfataricus
1.1.1.1 25.5
-
 benzaldehyde mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
1.1.1.1 25.5
-
 4-methoxybenzyl alcohol mutant enzyme N249Y, at 65°C Saccharolobus solfataricus
1.1.1.1 50
-
 NAD+ mutant enzyme N249Y, at 65°C, in the presence of benzyl alcohol Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 6.9 7.5 apparent optimal pH for the benzaldehyde reduction Saccharolobus solfataricus
1.1.1.1 8.5
-
 mutant enzyme N249Y Saccharolobus solfataricus
1.1.1.1 9
-
 wild type enzyme Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+ dependent Saccharolobus solfataricus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.1 1.7
-
 wild type enzyme Saccharolobus solfataricus guanidine hydrochloride
1.1.1.1 2.2
-
 mutant enzyme N249Y Saccharolobus solfataricus guanidine hydrochloride