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Literature summary extracted from
- Phosphatidylcholine/sphingomyelin metabolism crosstalk inside the nucleus (2008), Biochem. J., 410, 381-389.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | phosphatidylcholine-specific phospholipase C | increases the N-SMase activity up to 2fold | Rattus norvegicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | D609 | 48% inhibition of the enzyme in chromain after 30 min of treatment, no inhibition by D609 in the nuclear membrane | Rattus norvegicus |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.4.12 | chromatin | - |
Rattus norvegicus | 785 | - |
| 3.1.4.12 | nuclear membrane | - |
Rattus norvegicus | 31965 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | Ca2+ | - |
Rattus norvegicus | |
| 3.1.4.12 | Mg2+ | - |
Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.12 | additional information | Rattus norvegicus | a phosphatidylcholine/sphingomyelin metabolism crosstalk which regulates the intranuclear ceramide/diacylglycerol pool exists in the chromatin structure, effect of phosphatidylcholine-specific phospholipase C activity on neutral sphingomyelinase and reverse sphingomyelin synthase, which enrich the intranuclear ceramide pool, overview | ? | - |
? | |
| 3.1.4.12 | sphingomyelin + H2O | Rattus norvegicus | - |
N-acylsphingosine + choline phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.12 | Rattus norvegicus | - |
Sprague-Dawley rats | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.12 | hepatocyte | - |
Rattus norvegicus | - |
| 3.1.4.12 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 0.000025 | - |
chromatin | Rattus norvegicus |
| 3.1.4.12 | 0.0009 | - |
nuclear membrane | Rattus norvegicus |
| 3.1.4.12 | 0.052 | - |
phospholipid pre-incubated nuclear membrane | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.12 | additional information | a phosphatidylcholine/sphingomyelin metabolism crosstalk which regulates the intranuclear ceramide/diacylglycerol pool exists in the chromatin structure, effect of phosphatidylcholine-specific phospholipase C activity on neutral sphingomyelinase and reverse sphingomyelin synthase, which enrich the intranuclear ceramide pool, overview | Rattus norvegicus | ? | - |
? | |
| 3.1.4.12 | sphingomyelin + H2O | - |
Rattus norvegicus | N-acylsphingosine + choline phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.12 | N-SMase | - |
Rattus norvegicus |
| 3.1.4.12 | neutral sphingomyelinase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 7.6 | - |
assay at, nuclear membranes | Rattus norvegicus |
| 3.1.4.12 | 8.4 | - |
assay at, chromatin | Rattus norvegicus |
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Release 2023.1 (January 2023)
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