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Literature summary extracted from

  • Buchanan, C.L.; Connaris, H.; Danson, M.J.; Reeve, C.D.; Hough, D.W.
    An extremely thermostable aldolase from Sulfolobus solfataricus with specificity for non-phosphorylated substrates (1999), Biochem. J., 343, 563-570.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.55 expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.55 Hydroxypyruvate
-
Saccharolobus solfataricus
4.1.2.55 additional information no decrease in activity in the presence of 50 mM EDTA Saccharolobus solfataricus
4.1.2.55 NaBH4 69% inactivation in the presence of NaBH% and pyruvate, complete inactivation when the reaction time is increased to 20 min Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.55 additional information activity is unaffected by the presence of 0.1 mM ZnCl2 Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.55 32980
-
4 * 32980, calculated from sequence Saccharolobus solfataricus
4.1.2.55 133000
-
equilibrium centrifugation analysis Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.55 Saccharolobus solfataricus O54288
-
-
4.1.2.55 Saccharolobus solfataricus DSM 1616 O54288
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.55
-
Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.55 additional information
-
1.1 U/mg, pH 6.0, 70°C Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.55 pyruvate + D-glyceraldehyde no activity could be detected with D-glyceraldehyde 3-phosphate as substrate in place of glyceraldehyde or with the pyruvate analogues beta-hydroxypyruvate and alpha-ketobutyrate. Studies by other groups reveal activity with D-glyceraldehyde 3-phosphate Saccharolobus solfataricus 2-dehydro-3-deoxy-D-gluconate
-
r
4.1.2.55 pyruvate + D-glyceraldehyde no activity could be detected with D-glyceraldehyde 3-phosphate as substrate in place of glyceraldehyde or with the pyruvate analogues beta-hydroxypyruvate and alpha-ketobutyrate. Studies by other groups reveal activity with D-glyceraldehyde 3-phosphate Saccharolobus solfataricus DSM 1616 2-dehydro-3-deoxy-D-gluconate
-
r

Subunits

EC Number Subunits Comment Organism
4.1.2.55 tetramer 4 * 32980, calculated from sequence Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
4.1.2.55 KDG-aldolase
-
Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.55 70
-
assay at Saccharolobus solfataricus
4.1.2.55 95
-
-
Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.2.55 70 100 70°C: about 40% of maximal activity, 100°C: about 95% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.55 95
-
half-life: 7.8 h Saccharolobus solfataricus
4.1.2.55 100
-
half-life: 2.5 h Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.55 6
-
assay at Saccharolobus solfataricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.2.55 0.3
-
Hydroxypyruvate pH 6.0, 70°C Saccharolobus solfataricus