EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.33 | - |
Mycobacterium tuberculosis |
4.1.1.36 | expressed in Escherichia coli BL21 (DE3) cells | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.33 | CoA | - |
Mycobacterium tuberculosis | |
4.1.1.36 | 4'-phosphopantothenol | - |
Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.33 | 0.05 | - |
ATP | 25°C, pH 7.8, determined by isothermal titration calorimetry | Mycobacterium tuberculosis | |
2.7.1.33 | 0.1 | - |
pantothenate | 25°C, pH 7.8, determined from coupled assay | Mycobacterium tuberculosis | |
2.7.1.33 | 0.18 | - |
pantothenate | 25°C, pH 7.8, determined by isothermal titration calorimetry | Mycobacterium tuberculosis | |
2.7.1.33 | 0.25 | - |
pantothenol | 25°C, pH 7.8, determined by isothermal titration calorimetry | Mycobacterium tuberculosis | |
2.7.1.33 | 0.28 | - |
pantothenol | 25°C, pH 7.8, determined from coupled assay | Mycobacterium tuberculosis | |
2.7.1.33 | 0.375 | - |
ATP | 25°C, pH 7.8, determined from coupled assay | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.33 | Mycobacterium tuberculosis | P9WPA7 | - |
- |
2.7.1.33 | Mycobacterium tuberculosis H37Rv | P9WPA7 | - |
- |
4.1.1.36 | Mycobacterium tuberculosis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.36 | Ni-NTA metal-affinity column chromatography | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.33 | ATP + pantothenate | - |
Mycobacterium tuberculosis | ADP + 4'-phosphopantothenate | - |
? | |
2.7.1.33 | ATP + pantothenate | - |
Mycobacterium tuberculosis H37Rv | ADP + 4'-phosphopantothenate | - |
? | |
2.7.1.33 | ATP + pantothenol | - |
Mycobacterium tuberculosis | ADP + 4'-phosphopantothenol | - |
? | |
2.7.1.33 | ATP + pantothenol | - |
Mycobacterium tuberculosis H37Rv | ADP + 4'-phosphopantothenol | - |
? | |
4.1.1.36 | N-[(R)-4'-phosphopantothenoyl]-L-cysteine | - |
Mycobacterium tuberculosis | pantotheine 4'-phosphate + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.33 | MtCoaA | - |
Mycobacterium tuberculosis |
4.1.1.36 | 4'-phosphopantothenoylcysteine decarboxylase | - |
Mycobacterium tuberculosis |
4.1.1.36 | CoaBC | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.33 | 0.38 | - |
pantothenol | 25°C, pH 7.8, determined by isothermal titration calorimetry | Mycobacterium tuberculosis | |
2.7.1.33 | 0.4 | - |
pantothenol | 25°C, pH 7.8, determined from coupled assay | Mycobacterium tuberculosis | |
2.7.1.33 | 0.61 | - |
pantothenate | 25°C, pH 7.8, determined from coupled assay | Mycobacterium tuberculosis | |
2.7.1.33 | 0.62 | - |
pantothenate | 25°C, pH 7.8, determined by isothermal titration calorimetry | Mycobacterium tuberculosis | |
2.7.1.33 | 0.62 | - |
ATP | 25°C, pH 7.8, determined from coupled assay | Mycobacterium tuberculosis | |
2.7.1.33 | 0.65 | - |
ATP | 25°C, pH 7.8, determined by isothermal titration calorimetry | Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.36 | flavin | - |
Mycobacterium tuberculosis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.33 | 0.002 | - |
CoA | - |
Mycobacterium tuberculosis |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
4.1.1.36 | 0.063 | - |
in 50 mM Tris, 0.2 mM cysteine, 0.2 mM CTP, 0.2 mM dithiothreitol, 0.2 mM MgCl2, at 37°C | Mycobacterium tuberculosis | 4'-phosphopantothenol |