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Literature summary extracted from

  • Kumar, P.; Chhibber, M.; Surolia, A.
    How pantothenol intervenes in coenzyme-A biosynthesis of Mycobacterium tuberculosis (2007), Biochem. Biophys. Res. Commun., 361, 903-909.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.33
-
Mycobacterium tuberculosis
4.1.1.36 expressed in Escherichia coli BL21 (DE3) cells Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.33 CoA
-
Mycobacterium tuberculosis
4.1.1.36 4'-phosphopantothenol
-
Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.33 0.05
-
ATP 25°C, pH 7.8, determined by isothermal titration calorimetry Mycobacterium tuberculosis
2.7.1.33 0.1
-
pantothenate 25°C, pH 7.8, determined from coupled assay Mycobacterium tuberculosis
2.7.1.33 0.18
-
pantothenate 25°C, pH 7.8, determined by isothermal titration calorimetry Mycobacterium tuberculosis
2.7.1.33 0.25
-
pantothenol 25°C, pH 7.8, determined by isothermal titration calorimetry Mycobacterium tuberculosis
2.7.1.33 0.28
-
pantothenol 25°C, pH 7.8, determined from coupled assay Mycobacterium tuberculosis
2.7.1.33 0.375
-
ATP 25°C, pH 7.8, determined from coupled assay Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Mycobacterium tuberculosis P9WPA7
-
-
2.7.1.33 Mycobacterium tuberculosis H37Rv P9WPA7
-
-
4.1.1.36 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.36 Ni-NTA metal-affinity column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 ATP + pantothenate
-
Mycobacterium tuberculosis ADP + 4'-phosphopantothenate
-
?
2.7.1.33 ATP + pantothenate
-
Mycobacterium tuberculosis H37Rv ADP + 4'-phosphopantothenate
-
?
2.7.1.33 ATP + pantothenol
-
Mycobacterium tuberculosis ADP + 4'-phosphopantothenol
-
?
2.7.1.33 ATP + pantothenol
-
Mycobacterium tuberculosis H37Rv ADP + 4'-phosphopantothenol
-
?
4.1.1.36 N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
Mycobacterium tuberculosis pantotheine 4'-phosphate + CO2
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.33 MtCoaA
-
Mycobacterium tuberculosis
4.1.1.36 4'-phosphopantothenoylcysteine decarboxylase
-
Mycobacterium tuberculosis
4.1.1.36 CoaBC
-
Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.33 0.38
-
pantothenol 25°C, pH 7.8, determined by isothermal titration calorimetry Mycobacterium tuberculosis
2.7.1.33 0.4
-
pantothenol 25°C, pH 7.8, determined from coupled assay Mycobacterium tuberculosis
2.7.1.33 0.61
-
pantothenate 25°C, pH 7.8, determined from coupled assay Mycobacterium tuberculosis
2.7.1.33 0.62
-
pantothenate 25°C, pH 7.8, determined by isothermal titration calorimetry Mycobacterium tuberculosis
2.7.1.33 0.62
-
ATP 25°C, pH 7.8, determined from coupled assay Mycobacterium tuberculosis
2.7.1.33 0.65
-
ATP 25°C, pH 7.8, determined by isothermal titration calorimetry Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.36 flavin
-
Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.33 0.002
-
CoA
-
Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.1.1.36 0.063
-
in 50 mM Tris, 0.2 mM cysteine, 0.2 mM CTP, 0.2 mM dithiothreitol, 0.2 mM MgCl2, at 37°C Mycobacterium tuberculosis 4'-phosphopantothenol