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Literature summary extracted from

  • Bhandare, R.; Calabro, M.; Coschigano, P.W.
    Site-directed mutagenesis of the Thauera aromatica strain T1 tutE tutFDGH gene cluster (2006), Biochem. Biophys. Res. Commun., 346, 992-998.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.99.11 additional information tutE gene product (benzylsuccinate synthase activating enzyme) required for activity, changes in the tutE encoded protein (C72A and C79A) are able to prevent the action of benzylsuccinate synthase Thauera aromatica
4.1.99.11 additional information tutH gene product required for activity, changes in the tutH encoded protein (G52A/K53R/S54A) are able to prevent the action of benzylsuccinate synthase, tutH gene product possibly involved in assembly of the enzyme subunits Thauera aromatica

Protein Variants

EC Number Protein Variants Comment Organism
4.1.99.11 C29S tutG gene, strong loss of activity, engineered strain unable to grow with toluene as carbon source Thauera aromatica
4.1.99.11 C9Y/A10S tutF gene, strong loss of activity, engineered strain unable to grow with toluene as carbon source Thauera aromatica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.11 toluene + fumarate Thauera aromatica first step in the anaerobic degradation pathway of toluene benzylsuccinate
-
 ?
4.1.99.11 toluene + fumarate Thauera aromatica T1 first step in the anaerobic degradation pathway of toluene benzylsuccinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.11 Thauera aromatica
-
 enzyme encoded as part of the tutE tutFDGH gene cluster
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4.1.99.11 Thauera aromatica T1
-
 enzyme encoded as part of the tutE tutFDGH gene cluster
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.1.99.11 extremely oxygen sensitive Thauera aromatica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.11 toluene + fumarate first step in the anaerobic degradation pathway of toluene Thauera aromatica benzylsuccinate
-
 ?
4.1.99.11 toluene + fumarate first step in the anaerobic degradation pathway of toluene Thauera aromatica T1 benzylsuccinate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.99.11 hexamer alpha2 beta2 gamma2, three subunits encoded by the genes tutF, tutD, and tutG Thauera aromatica