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Literature summary extracted from

  • Hansen, T.; Urbanke, C.; Leppänen, V.M.; Goldman, A.; Brandenburg, K.; Schäfer, G.
    The extreme thermostable pyrophosphatase from Sulfolobus acidocaldarius: enzymatic and comparative biophysical characterization (1999), Arch. Biochem. Biophys., 363, 135-147.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.1 expression in Escherichia coli Sulfolobus acidocaldarius

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.1 CD and FTIR spectra demonstrate a similar overall fold for enzyme and PPases from Escherichia coli and Thermus thermophilus Sulfolobus acidocaldarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.1 0.0054
-
 diphosphate pH 7.0, 75°C Sulfolobus acidocaldarius

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.1 Ca2+ 2% of the activity with Mg2+ Sulfolobus acidocaldarius
3.6.1.1 Co2+ 88% of the activity with Mg2+ Sulfolobus acidocaldarius
3.6.1.1 Mg2+ preferred divalent cation, Km value 0.9 mM Sulfolobus acidocaldarius
3.6.1.1 Mn2+ 32% of the activity with Mg2+ Sulfolobus acidocaldarius
3.6.1.1 additional information divalent cation required. Activity in decreasing order: Mg2+, Zn2+, Co2+, Mn2+, Ni2+, Ca2+ Sulfolobus acidocaldarius
3.6.1.1 Ni2+ 3% of the activity with Mg2+ Sulfolobus acidocaldarius
3.6.1.1 Zn2+ 95% of the activity with Mg2+ Sulfolobus acidocaldarius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.1 19365
-
 x * 19365, calculated Sulfolobus acidocaldarius

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Sulfolobus acidocaldarius
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.1 860
-
 pH 7.0, 75°C Sulfolobus acidocaldarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 ADP + H2O 3-6% of the activity with diphosphate Sulfolobus acidocaldarius ? + phosphate
-
 ?
3.6.1.1 ATP + H2O 3-6% of the activity with diphosphate Sulfolobus acidocaldarius ? + phosphate
-
 ?
3.6.1.1 diphosphate + H2O
-
 Sulfolobus acidocaldarius 2 phosphate
-
 ?
3.6.1.1 ITP + H2O 10% of the activity with diphosphate Sulfolobus acidocaldarius ? + phosphate
-
 ?
3.6.1.1 phosphoenolpyruvate + H2O 1-2% of the activity with diphosphate Sulfolobus acidocaldarius pyruvate + phosphate
-
 ?
3.6.1.1 triphosphate + H2O 3-6% of the activity with diphosphate Sulfolobus acidocaldarius ? + phosphate
-
 ?
3.6.1.1 TTP + H2O 10% of the activity with diphosphate Sulfolobus acidocaldarius ? + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.1 ? x * 19365, calculated Sulfolobus acidocaldarius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.1 89
-
 melting temperature, presence of EDTA Sulfolobus acidocaldarius
3.6.1.1 95
-
 half-life of catalytic activity 2.5 h Sulfolobus acidocaldarius
3.6.1.1 99
-
 melting temperature, presence of Mg2+ Sulfolobus acidocaldarius
3.6.1.1 100
-
 melting temperature above 100°C, prensence of Mn2+ Sulfolobus acidocaldarius

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.1 1700
-
 diphosphate pH 7.0, 75°C Sulfolobus acidocaldarius

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.6.1.1 4.5 9
-
 Sulfolobus acidocaldarius