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Literature summary extracted from
- Complete hydrolysis of myo-inositol hexakisphosphate by a novel phytase from Debaryomyces castellii CBS 2923 (2008), Appl. Microbiol. Biotechnol., 78, 47-53.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.8 | Ca2+ | 5 mM, 10% decreased activity, although calcium has no direct influence on enzymatic activity, it plays a crucial protective role, since in the absence of calcium, the denaturation of the enzyme is complete after 1 h at 66.5°C | Debaryomyces castellii |  |
| 3.1.3.8 | Fe2+ | 5 mM, 10% decreased activity | Debaryomyces castellii | |
| 3.1.3.8 | Mn2+ | 5 mM, 71% decreased activity | Debaryomyces castellii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.8 | 0.532 | - |
myo-inositol hexakisphosphate | in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0 at 37°C | Debaryomyces castellii | |
| 3.1.3.8 | 2.27 | - |
p-nitrophenyl phosphate | in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0 at 37°C | Debaryomyces castellii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 51000 | - |
SDS-PAGE, after deglycosylation | Debaryomyces castellii |
| 3.1.3.8 | 52980 | - |
mass spectrometry analysis, after deglycosylation | Debaryomyces castellii |
| 3.1.3.8 | 74460 | - |
mass spectrometry analysis, with 28.8% glycosylation | Debaryomyces castellii |
| 3.1.3.8 | 77000 | - |
SDS-PAGE, with 28.8% glycosylation | Debaryomyces castellii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.8 | Debaryomyces castellii | - |
- |
- |
| 3.1.3.8 | Debaryomyces castellii CBS 2923 | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | glycoprotein | 28.8% glycosylation | Debaryomyces castellii |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.8 | HiPrep Phenyl FF column chromatography | Debaryomyces castellii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 157.4 | - |
purified extract, at pH 4.0 | Debaryomyces castellii |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.3.8 | 60°C, in water, 1 h, remains stable | Debaryomyces castellii |
| 3.1.3.8 | 66°C, in sodium acetate buffer (125 mM, pH 4.0), 1 h, remains stable | Debaryomyces castellii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.8 | 3-phosphoglycerate + H2O | 95% activity compared to myo-inositol hexakisphosphate | Debaryomyces castellii | glycerate + phosphate | - |
? | |
| 3.1.3.8 | 3-phosphoglycerate + H2O | 95% activity compared to myo-inositol hexakisphosphate | Debaryomyces castellii CBS 2923 | glycerate + phosphate | - |
? | |
| 3.1.3.8 | ADP + H2O | 120% activity compared to myo-inositol hexakisphosphate | Debaryomyces castellii | AMP + phosphate | - |
? | |
| 3.1.3.8 | alpha-glycerophosphate + H2O | 78% activity compared to myo-inositol hexakisphosphate | Debaryomyces castellii | glycerol + phosphate | - |
? | |
| 3.1.3.8 | AMP + H2O | 50% activity compared to myo-inositol hexakisphosphate | Debaryomyces castellii | adenosine + phosphate | - |
? | |
| 3.1.3.8 | AMP + H2O | 50% activity compared to myo-inositol hexakisphosphate | Debaryomyces castellii CBS 2923 | adenosine + phosphate | - |
? | |
| 3.1.3.8 | ATP + H2O | 133% activity compared to myo-inositol hexakisphosphate | Debaryomyces castellii | ADP + phosphate | - |
? | |
| 3.1.3.8 | D-fructose 6-phosphate + H2O | 35% activity compared to myo-inositol hexakisphosphate | Debaryomyces castellii | D-fructose + phosphate | - |
? | |
| 3.1.3.8 | D-fructose 6-phosphate + H2O | 35% activity compared to myo-inositol hexakisphosphate | Debaryomyces castellii CBS 2923 | D-fructose + phosphate | - |
? | |
| 3.1.3.8 | D-glucose 6-phosphate + H2O | 82% activity compared to myo-inositol hexakisphosphate | Debaryomyces castellii | D-glucose + phosphate | - |
? | |
| 3.1.3.8 | inositol 1-monophosphate + H2O | 58% activity compared to myo-inositol hexakisphosphate | Debaryomyces castellii | inositol + phosphate | - |
? | |
| 3.1.3.8 | inositol 2-monophosphate + H2O | 84% activity compared to myo-inositol hexakisphosphate | Debaryomyces castellii | inositol + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | 100% activity | Debaryomyces castellii | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | p-nitrophenyl phosphate + H2O | 135% activity compared to myo-inositol hexakisphosphate | Debaryomyces castellii | p-nitrophenol + phosphate | - |
? | |
| 3.1.3.8 | p-nitrophenyl phosphate + H2O | 135% activity compared to myo-inositol hexakisphosphate | Debaryomyces castellii CBS 2923 | p-nitrophenol + phosphate | - |
? | |
| 3.1.3.8 | phosphoenolpyruvate + H2O | 130% activity compared to myo-inositol hexakisphosphate | Debaryomyces castellii | pyruvate + phosphate | - |
? | |
| 3.1.3.8 | phosphoenolpyruvate + H2O | 130% activity compared to myo-inositol hexakisphosphate | Debaryomyces castellii CBS 2923 | pyruvate + phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | 3-phytase | - |
Debaryomyces castellii |
| 3.1.3.8 | myo-inositol hexakisphosphate 3-phosphohydrolase | - |
Debaryomyces castellii |
| 3.1.3.8 | PhyA | - |
Debaryomyces castellii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 55 | 60 | - |
Debaryomyces castellii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 70 | - |
70% loss of activity after 1 h at 70°C | Debaryomyces castellii |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.8 | 44.2 | - |
p-nitrophenyl phosphate | in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C | Debaryomyces castellii | |
| 3.1.3.8 | 51.2 | - |
myo-inositol hexakisphosphate | in 200 mM sodium acetate buffer, 1 mM CaCl2, pH 4.0, at 37°C | Debaryomyces castellii | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 4 | 4.5 | - |
Debaryomyces castellii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 2.5 | 6.5 | - |
Debaryomyces castellii |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 3 | 7 | no denaturation is observed for pH values between 5.0 and 7.0 after 67 days of storage at -20°C, whereas the phytase is denatured for pH values lower than 5.0, for pH values ranging from 3.0 to 7.0, the enzyme retains 80 to 100% of its activity after incubation at 40°C for 1 h | Debaryomyces castellii |
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