EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.1 | expression in Escherichia coli, with c-terminal His-tag and wizthout the N-terminal hydrophobic region | Thermotoga maritima |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.1 | 2-mercaptoethanol | 94% residual activity | Thermotoga maritima | |
3.1.1.1 | diethyl dicarbonate | - |
Thermotoga maritima | |
3.1.1.1 | EDTA | 81% residual activity | Thermotoga maritima | |
3.1.1.1 | EGTA | 39% residual activity | Thermotoga maritima | |
3.1.1.1 | Mn2+ | 5 mM, 62% of initial activity | Thermotoga maritima | |
3.1.1.1 | Ni2+ | 5 mM, 67% of initial activity | Thermotoga maritima | |
3.1.1.1 | O,O'-bis(2-aminoethyl)ethylenglycol-N,N,N',N'-tetraacetic acid | - |
Thermotoga maritima | |
3.1.1.1 | Phenylmethyl sulfonylfluoride | 11% residual activity | Thermotoga maritima | |
3.1.1.1 | phenylmethylsulfonyl fluoride | - |
Thermotoga maritima | |
3.1.1.1 | Zn2+ | 5 mM, 41% of initial activity | Thermotoga maritima |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.1 | 0.00031 | - |
p-nitrophenyl decanoate | 60°C, pH 7.5 | Thermotoga maritima | |
3.1.1.1 | 0.0021 | - |
p-nitrophenyl myristate | 60°C, pH 7.5 | Thermotoga maritima | |
3.1.1.1 | 0.0047 | - |
p-nitrophenyl caprylate | 60°C, pH 7.5 | Thermotoga maritima | |
3.1.1.1 | 0.243 | - |
p-nitrophenyl butanoate | 60°C, pH 7.5 | Thermotoga maritima | |
3.1.1.1 | 1.15 | - |
p-nitrophenyl acetate | 60°C, pH 7.5 | Thermotoga maritima |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.1 | Ca2+ | 5 mM, 236% of initial activity | Thermotoga maritima | |
3.1.1.1 | Fe2+ | 5 mM, 196% of initial activity | Thermotoga maritima | |
3.1.1.1 | K+ | 5 mM, 137% of initial activity | Thermotoga maritima | |
3.1.1.1 | Mg2+ | 5 mM, 214% of initial activity | Thermotoga maritima |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.1 | 39313 | - |
x * 40000, SDS-PAGE, x * 39313, calculated, His-tagged protein | Thermotoga maritima |
3.1.1.1 | 40000 | - |
x * 40000, SDS-PAGE, x * 39313, calculated, His-tagged protein | Thermotoga maritima |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.1.1.1 | dimethyl sulfoxide | 5%, 146% of initial activity | Thermotoga maritima |
3.1.1.1 | dimethylformamide | 5%, 99% of initial activity | Thermotoga maritima |
3.1.1.1 | dioxane | 5%, 141% of initial activity | Thermotoga maritima |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.1 | Thermotoga maritima | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.1 | recombinant protein | Thermotoga maritima |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.1 | 4-nitrophenyl decanoate + H2O | - |
Thermotoga maritima | 4-nitrophenol + decanoate | - |
? | |
3.1.1.1 | p-nitrophenyl acetate + H2O | - |
Thermotoga maritima | p-nitrophenol + acetate | - |
? | |
3.1.1.1 | p-nitrophenyl butanoate + H2O | - |
Thermotoga maritima | p-nitrophenol + butanoate | - |
? | |
3.1.1.1 | p-nitrophenyl caprylate + H2O | - |
Thermotoga maritima | p-nitrophenol + caprylate | - |
? | |
3.1.1.1 | p-nitrophenyl decanoate | - |
Thermotoga maritima | p-nitrophenol + octanoate | - |
? | |
3.1.1.1 | p-nitrophenyl myristate + H2O | - |
Thermotoga maritima | p-nitrophenol + myristate | - |
? | |
3.1.1.1 | p-nitrophenyl palmitate + H2O | 50% of the activity with p-nitrophenyl decanoate | Thermotoga maritima | p-nitrophenol + palmitate | - |
? | |
3.1.1.1 | p-nitrophenyl stearate + H2O | 50% of the activity with p-nitrophenyl decanoate | Thermotoga maritima | p-nitrophenol + stearate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.1 | ? | x * 40000, SDS-PAGE, x * 39313, calculated, His-tagged protein | Thermotoga maritima |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.1 | 60 | - |
- |
Thermotoga maritima |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.1 | 60 | - |
90 min, stable | Thermotoga maritima |
3.1.1.1 | 80 | - |
30 min, 50% residual activity | Thermotoga maritima |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.1 | 4.9 | - |
p-nitrophenyl myristate | 60°C, pH 7.5 | Thermotoga maritima | |
3.1.1.1 | 10.8 | - |
p-nitrophenyl decanoate | 60°C, pH 7.5 | Thermotoga maritima | |
3.1.1.1 | 13 | - |
p-nitrophenyl caprylate | 60°C, pH 7.5 | Thermotoga maritima | |
3.1.1.1 | 14 | - |
p-nitrophenyl butanoate | 60°C, pH 7.5 | Thermotoga maritima | |
3.1.1.1 | 50 | - |
p-nitrophenyl acetate | 60°C, pH 7.5 | Thermotoga maritima |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.1 | 6.5 | 7 | - |
Thermotoga maritima |