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Literature summary extracted from

  • Wang, N.C.; Lee, C.Y.
    Molecular cloning of the aspartate 4-decarboxylase gene from Pseudomonas sp. ATCC 19121 and characterization of the bifunctional recombinant enzyme (2006), Appl. Microbiol. Biotechnol., 73, 339-348.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.12 2-mercaptoethanol 1 mM, 7% activation Pseudomonas sp.
4.1.1.12 DTT 1 mM, 7% activation Pseudomonas sp.
4.1.1.12 EDTA 10-20 mM, 10% activation Pseudomonas sp.

Application

EC Number Application Comment Organism
4.1.1.12 industry Asd is a major enzyme used in the industrial production of L-alanine Pseudomonas sp.
4.1.1.12 synthesis Asd is a major enzyme used in the industrial production of L-alanine Pseudomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.12 gene asd, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of the C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.12 Ca2+ 10 mM, 14% inhibition Pseudomonas sp.
4.1.1.12 Co2+ 10 mM, over 90% inhibition Pseudomonas sp.
4.1.1.12 Cu2+ 10 mM, over 90% inhibition Pseudomonas sp.
4.1.1.12 Fe2+ 10 mM, over 90% inhibition Pseudomonas sp.
4.1.1.12 Hg2+ 10 mM, complete inhibition Pseudomonas sp.
4.1.1.12 Mg2+ 10 mM, 64% inhibition Pseudomonas sp.
4.1.1.12 Mn2+ 10 mM, over 90% inhibition Pseudomonas sp.
4.1.1.12 Ni2+ 10 mM, over 90% inhibition Pseudomonas sp.
4.1.1.12 Zn2+ 10 mM, over 90% inhibition Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.12 11.5
-
 L-aspartate pH 6.8, 37°C, recombinant enzyme Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.12 additional information no divalent metal ion required for catalysis Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.12 59243
-
 12 * 63000, pH 7.0, SDS-PAGE, 12 * 59243, sequence calculation Pseudomonas sp.
4.1.1.12 59243
-
 2 * 63000, pH 7.0, SDS-PAGE, 2 * 59243, sequence calculation Pseudomonas sp.
4.1.1.12 63000
-
 12 * 63000, pH 7.0, SDS-PAGE, 12 * 59243, sequence calculation Pseudomonas sp.
4.1.1.12 63000
-
 2 * 63000, pH 7.0, SDS-PAGE, 2 * 59243, sequence calculation Pseudomonas sp.
4.1.1.12 760000
-
 dodedamer, pH 5.0, recombinant enzyme, gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.12 L-aspartate Pseudomonas sp.
-
 L-alanine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.12 Pseudomonas sp. Q53IZ1 strain ATCC 19121
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.12 recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.12 280
-
 purified recombinant enzyme, pH 5.0, 37°C Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.12 L-aspartate
-
 Pseudomonas sp. L-alanine + CO2
-
 ?
4.1.1.12 L-aspartate the decarboxylation activity is 2477fold higher than the aminotransferase activity of the enzyme, highly preferred substrate Pseudomonas sp. L-alanine + CO2
-
 ?
4.1.1.12 additional information D-aspartate, L-lysine, L-asparagine, L-glutamate, and L-glutamine are poor substrates in beta-elimination and transamination reaction catalyzes by the enzyme, substrate specificity, overview Pseudomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.12 dimer 2 * 63000, pH 7.0, SDS-PAGE, 2 * 59243, sequence calculation Pseudomonas sp.
4.1.1.12 dodecamer 12 * 63000, pH 7.0, SDS-PAGE, 12 * 59243, sequence calculation Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
4.1.1.12 Asd
-
 Pseudomonas sp.
4.1.1.12 L-aspartate 4-decarboxylase
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.12 45
-
-
 Pseudomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.12 40 45 92% of maximal activity at 40°C, maximal activity at 45°C Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.12 5
-
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.12 4 8.5 purified recombinant enzyme, 4°C, 1 day, completely stable Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.12 pyridoxal 5'-phosphate dependent on Pseudomonas sp.